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http://dx.doi.org/10.7231/JON.2012.23.1.129

Neuroprotective effects of Sohaphwangwon essential oil in a Parkinson's disease mouse model  

Kim, In-Ja (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University)
Lee, Ji-Hyun (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University)
Song, Kyoo-Ju (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University)
Koo, Byung-Soo (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University)
Kim, Geun-Woo (Dept. of Neuropsychiatry, College of Oriental Medicine, Dong-Guk University)
Publication Information
Journal of Oriental Neuropsychiatry / v.23, no.1, 2012 , pp. 129-143 More about this Journal
Abstract
Objectives : To evaluate the neuroprotective effects of the essential oil from Sohaphwangwon (SH), a Chinese traditional medicinal prescription in a Parkinson's disease mouse model. Methods : 1. The neuroprotective effect of SH on primary neuronal cells was examined by using 1-methyl-4-phenylpyridinium ion (MPP+). 2. The neuroprotective effect of SH was examined in a Parkinson's disease mouse model. C57BL/6 mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg/day), intraperitoneal (i.p.) for 5 days. SH inhalation was applied before MPTP treatment for 7 days and continued until 12 days after the first MPTP treatment. 3. To find out the intracellular target signal molecule(s) regarding the neuroprotective effect of SH essential oil, brain-derived neurotropic factor (BDNF) and synaptic protein SNAP25 were examined by Western blot analysis. Results : 1. MPP+ induced a concentration-dependent decrease in cell viability. However, in the presence of 3 and 5 ug/ml of SH, MPP+-induced cell death was significantly reduced. 2. SH inhalation in MPTP mice led to the restoration of behavioral impairment and rescued tyrosine hydroxylase (TH)-positive dopaminergic neurodegeneration. 3. In SH / MPTP mice, BDNF and SNAP25 increased. Conclusions : This experiment suggests that the neuroprotective effect of SH essential oil is mediated by the expression of BDNF. Furthermore, SH essential oil may serve as a potential preventive or therapeutic agent regarding Parkinson's disease.
Keywords
Sohaphwangwon; Parkinson's disease; 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP);
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