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Up-and-Down Procedure(UDP) Determinations of Acute Oral Toxicity of LMK02-Jangwonhwan in SD Rats  

Kang, Hyung-Won (Dept. of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University)
Kwon, Yeong-Mi (Dept. of Diagnostic Radiology, College of Oriental Medicine Won-Kwang University)
Lee, Sang-Won (National Institute of Horticultural and Herbal Science)
Kim, Ji-Hwon (Dept. of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University)
Lee, Hyo-Gyung (Dept. of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University)
Jang, Hyun-Ho (Dept. of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University)
Park, Bo-Ra (Dept. of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University)
Lyu, Yeoung-Su (Dept. of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University)
Publication Information
Journal of Oriental Neuropsychiatry / v.21, no.3, 2010 , pp. 87-93 More about this Journal
Abstract
Objectives : The oriental medicine Jangwonhwan originally described in the Korean medical text, DonguiBogam(amnesia chapter). Recently, a modified formula of Jangwonhwan(LMK02-Jangwonhwan), was shown to reduce $\beta$-amyloid deposition in the brain of Tg-APPswe/PS1dE9 mouse model for Alzheimer's disease. This experiment aimed to investigate the acute oral toxicity of LMK02 in SD rats by up-and-down procedure determinations. Methods : Quality control of tablet form of LMK02 was established by estimating indicative components, Ginsenoside Rg3 of Red Ginseng and Decursin of Angelicagigas Nakai. The toxicity of LMK02 was investigated in 6 week old, specific pathogen free(SPF), Sprageu-Dawley rats. 3 female rats received 5,000 mg/10 ml/kg of test substance and their death rate, clinical sings, weight changes and autopsy findings had been observed for 2 weeks. Results : Any specific symptoms or death were resulted in this experiment. No significant changes in rats' weight. No significant differences in atopsy. Conclusions : The minimum lethal dose(MLD) of LMK02 for female Sprauge-Dawley rats were more than 5,000mg/kg in this experiment.
Keywords
Oriental medicine; Alzheimer disease; LMK02-Jangwonhwan; Toxicity test;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
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1 박용훈, 손일홍, 이상원, 임경헌, 김태헌, 류영수, 강형원. $H_2O_2$로 유도된 산화적 스트레스에 대한 壯元丸加減方의 PC12 cell에서의 항산화효과. 동의신경정신과학회지. 2009;20(2):19-29.   과학기술학회마을
2 최혁, 김상호, 이대용, 안대종, 강형원, 류영수 : pCT105로 유도된 치매모델에서 石菖蒲 수 추출액이 미치는 영향. 동의신경정신과학회지. 2002;3(2):173-94.   과학기술학회마을
3 Research, "Development of Functional foods for the Prevention of the circulatory dieseases and dementia using agricultural and fishery resources" the Ministry of agriculture and forestry. korea. 1998.
4 장순애. 참당귀(Angelica gigas Nakai)추출물로부터 아세틸콜린에스테라아제 저해 활성을 갖는 물질 분리 및 치매모델 마우스의 뇌 조직 활성에 미치는 영향. 대전 충남대대학원 박사. 2009.
5 김진우, 이순이, 이종화, 민상준, 김태헌, 류영수, 강형원. 玄蔘 수추출물이 아밀로이드 전구단백질로 형질전환된 초파리에 미치는 효과. 동의신경정신과학회지. 2009;20(2):121-31.   과학기술학회마을
6 Korea Food and Drug Administration. Good Laboratory Practice Regulation For Nonclinical Laboratory Studies, Notification No. 2005-79, Toxicity Test Guidelines for Safety Evaluation of Drugs.
7 OECD Guidelines for the Testing of Chemicals, Acute Oral Toxicity: Up-and-Down-Procedure (UDP). 2006:425.
8 신민교. 임상본초학. 서울:영림사. 2000:188.
9 김건진, 정대규. 壯元丹이 CT105 및 A$\beta$로 유발된 치매병태모델에 미치는 영향. 동의신경정신과학회지. 2006;17(2):91-122.   과학기술학회마을
10 강형원, 김상태, 이종화, 김태헌, 손형진, 한평림, 류영수. 壯元丸加減方 수추출물이 아밀로이드 전구단백질로 유도된 생쥐의 신경아세포주에서의 항치매 효과. 동의신경정신과학회지. 2007;18(2):13-24.   과학기술학회마을
11 김창민, 신민교, 안덕균, 이경순 외. 완역중약대사전. 서울:정담. 1999.
12 김영준, 김진형, 윤종현, 정은영, 김태현, 유영수, 강형원. 紅蔘 수추출물이 치매단백질 APP 형질전환 초파리에 미치는 영향. 동의신경정신과학회지. 2009;20(1):235-47.   과학기술학회마을
13 류성진. 한약재로부터 뇌세포 산화억제와 항허혈 치매물질 탐색. 인제대학교 대학원. 2003.
14 Korea Food and Drug Administration. Toxicity Test Guidelines for Safety Evaluation of Drugs, Notification. 2005:60.
15 許俊. 東醫寶鑑. 서울:法仁文化社. 1999:189-91.
16 강형원, 김상태, 손형진, 한평림, 조형권, 이영재, 류영수. LMK02의 품질규격화와 A$\beta$ oligomer에 의해 유도된 흰쥐해마 H19-7 세포주에 미치는 항치매 효과. 동의생리병리학회지. 2009; 23(2):397-404.   과학기술학회마을
17 Seo JS, Yun JH, Baek IS, Leem YH, Kang HW, Cho HK, Lyu YS, Son HJ, Han PL. Oriental medicine Jangwonhwan reduces Abeta(1-42) level and beta-amyloid deposition in the brain of Tg-APPswe/PS1dE9 mouse model of Alzheimer disease. J Ethnopharmacol. 2010; 128(1):206-12.   DOI   ScienceOn