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Effect of Chongmyung-Tang Prescription Combination on the Production of Amyloid β protein and β-site amyloid precursor protein-cleaving enzyme Activity in vitro  

Lim, Jung-Hwa (Dept. of Neuropsychiatry, Pu-San National University Korean Medicine Hospital)
Jung, In-Chul (Dept. of Neuropsychiatry, College of Oriental Medicine, Dae-Jeon University)
Lim, Jong-Soon (Dept. of Life Science and Preventative Medicine College of Oriental Medicine, Dae-Jeon University)
Kim, Seung-Hyung (Institute of Traditional Medicine and Bioscience, Dae-Jeon University)
Lee, Sang-Ryong (Dept. of Neuropsychiatry, College of Oriental Medicine, Dae-Jeon University)
Publication Information
Journal of Oriental Neuropsychiatry / v.21, no.2, 2010 , pp. 191-200 More about this Journal
Abstract
Objectives : This experiment was designed to investigate the effect of Chongmyung-Tang Prescription Combination(CmTP-$C_{1-10}$) extract on the production of amyloid $\beta$ protein and $\beta$-site amyloid precursor protein-cleaving enzyme(BACE) activity. Methods : The effect of CmTP-$C_{1-10}$ extract on expression of APP mRNA, BACE2 mRNA in BV2 microglia cell line treated by lipopolysacchride(LPS) and amyloid $\beta$ protein fragment(A$\beta$ fragment) were investigated. The effect of CmTP-$C_{1-10}$ extract on production of amyloid $\beta$ protein(A$\beta$) in BV2 microglia cell line treated by LPS and A$\beta$ fragment were investigated. The effect of CmTP-$C_{1-10}$ extract on BACE activity were investigated. Results : 1. CmTP-$C_9$ extract the most significantly suppressed the expression of APP mRNA, BACE2 mRNA in BV2 microglia cell line treated by LPS and A$\beta$ fragment. 2. CmTP-$C_9$ extract significantly suppressed the production of A$\beta$ in BV2 microglia cell line treated by LPS and A$\beta$ fragment. 3. CmTP-$C_9$ extract the most significantly inhibited BACE activity. Conclusions : These results suggest that CmTP-$C_9$ may be effective for the prevention and treatment of Alzheimer's Disease. Investigation into clinical use of CmTP-$C_9$ for Alzheimer's Disease is suggested for future research.
Keywords
Alzheimer's disease; Amyloid $\beta$ protein(A$\beta$); $\beta$-site amyloid precursor protein-cleaving enzyme(BACE); Chongmyung-Tang (C$\bar{o}$ngmingt$\bar{a}$ng) Prescription Combination(CmTP-$C_{1-10}$);
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