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A Study on the Effects of the Electro-Acupuncture(EA) Stimulation after Global Ischemia(GI) on Changes in Bax and Caspase-3 Expression among Forebrain Apoptosis  

Choi, Jung-Hyun (Dept. of Physical Therapy, Nam-Seoul Univ of Korea)
Min, Kyoung-Ok (Dept. of Physical Therapy, Yong-In Univ of Korea)
Kim, Soon-Hee (Dept. of Physical Therapy, Yong-In Univ of Korea)
Kim, Dong-Il (Dept. of Oriental Medicine Graduate School of Dongguk Univ.)
Lee, Sang-Bin (Dept. of Physical Therapy, Nam-Seoul Univ of Korea)
An, Ho-Jung (Dept. of Physical Therapy, Dae-Won Univ College of Korea)
Kim, Ji-Sung (Dept. of Physical Therapy, Suwon Women's College of Korea)
Choi, Wan-Suk (Dept. of Physical Therapy, Po-Hang College of Korea)
Kim, Bo-Kyoung (Dept. of Physical Therapy, Gun-Jang College of Korea)
Song, Chi-Won (Div. of General Service, National Institute of Food & Drug Safety Evaluation)
Publication Information
Journal of Oriental Neuropsychiatry / v.21, no.1, 2010 , pp. 59-69 More about this Journal
Abstract
Objectives: The study aims to look into how the application of EA(Electro-acupuncture) in the initial step of ischemic brain injury affects the changes in Bax and Caspase-3 expression among forebrain apoptosis. Methods: It caused GI by using common carotid artery occlusion and conducted the test by dividing into the no applied EA group after 6 hours and 12 hours, the LI 4 EA group that applied EA to LI4, and GV 20 EA group that was applied to GV 20. After that, the following results were obtained by comparing Bax and Caspase-3 expression, which were apoptosis-related factors, with its application time through immunohistochemical staining after extracting brain of each group. Results: According to each group's Bax expression by the application time, the order of expression increase after 6 hours is shown to be the no applied EA group, LI 4 group, and GV 20 group. The stimulation after 12 hours was most lowly expressed in the GV 20 group, and the no applied EA group and LI 4 group showed similar level. Conclusions: EA stimulation in the initial step after ischemia seems to affect positively Bax and Caspase-3 expression that are index of forebrain apoptosis.
Keywords
Global ischemia; Electrical acupuncture; Bax; Caspase-3;
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