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The Effects of Moxifloxacin in Endotoxin-induced Acute Lung Injury  

Baek, Jong Hyun (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Yeungnam University)
Lee, Jang Hoon (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Yeungnam University)
Lee, Jung Cheul (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Yeungnam University)
Lee, Dong Hyup (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Yeungnam University)
Moon, Yong Suk (Department of Anatomy, College of Medicine, Catholic University of Daegu)
Lee, Young Man (Department of Physiology, College of Medicine, Catholic University of Daegu)
Publication Information
Journal of Chest Surgery / v.42, no.1, 2009 , pp. 1-8 More about this Journal
Abstract
Background: The pathophysiology of acute respiratory distress syndrome with sepsis is acute lung injury (ALI) that's' caused by endotoxin (LPS). We evaluate effects of moxifloxacin on LPS-induced ALI in a rat model. Material and Method: The rats were divided into 3 groups as the control group (C), the LPS insult group (L), and the LPS+moxifloxacin treated group (L-M). ALI was induced by endotracheal instillation of E.coli LPS, then moxifloxacin was given in 30 minutes. Five hours later, we checked the lung weight/body weight ratio(the L/BW ratio), the protein & neutrophils in the bronchoalveolar lavage fluid (BALF), the myeloperoxidase (MPO) activity & the malondialdehyde (MDA) content, the expressions of cytosolic and secretory phospholipase $A_2$ (c, $sPLA_2$), and the morphology of the lung with using a light microscope. Result: The L/BW ratio, the protein content and the neutrophil count in the BALF, and the MPO activity and the MDA content in lung were significantly increased in group L compared to group C, and these factors were markedly decreased in group L-M compare to group L. The $cPLA_2$ expression and the $sPLA_2$ expression were increased in group L and the $cPLA_2$ expression was decreased in group L-M. Yet the $sPLA_2$ expression was not changed in group L-M. Morphologically, many inflammatory findings were observed in group L, but not in group L-M. Conclusion: Many of the inflammatory changes of ALI that were caused by LPS insult were ameliorated by moxifloxacin treatment.
Keywords
Acute respiratory distress syndrome; Neutrophils; Phospholipase A;
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