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In Vitro Effects of Nitroglycerin, Nicardipine, Verapamil, and Papaverine on Rabbit Brachial and Celiac Arterial Tone  

Shinn, Sung-Ho (Department of Thoracic and Cardiovascular Surgery, Hanyang University Guri Hospital, Hanyang University College of Medicine)
Kim, Young-Hak (Department of Thoracic and Cardiovascular Surgery, Hanyang University Hospital, Hanyang University College of Medicine)
Seo, Jung-Kuk (Department of Anesthesiology, Hanyang University Hospital, Hanyang University College of Medicine)
Kim, Jin-Hyuk (Department of Physiology, Hanyang University College of Medicine)
Chung, Won-Sang (Department of Thoracic and Cardiovascular Surgery, Hanyang University Hospital, Hanyang University College of Medicine)
Jeon, Yang-Bin (Department of Thoracic and Cardiovascular Surergy, Gil Hospital, Gachon University of Medicine and Science)
Chang, Byung-Chul (Department of Thoracic and Cardiovascular Surgery, Severance Hospital, Yonsei University College of Medicine)
Jang, Hyo-Jun (Department of Thoracic and Cardiovascular Surgery, Hanyang University Hospital, Hanyang University College of Medicine)
Publication Information
Journal of Chest Surgery / v.41, no.5, 2008 , pp. 541-549 More about this Journal
Abstract
Background: Vasoconstrictor-induced reduction in arterial graft diameter can cause significant flow deprivation. The aim of this study was to evaluate the effect of vasodilator pretreatment on vasoconstrictor-induced blood vessel spasm in vitro. Material and Method: Rabbit brachial arteries (BA) and celiac arteries (CA) were cut into rings $(3{\sim}4mm)$ and suspended with a force displacement transducer (TSD $125C^{(R)}$, Biopac Inc. USA) in a tissue bath filled with 5 mL modified Krebs solution bubbled with 5% $CO_2$ and 95% $O_2\;at\;38^{\circ}C$. The rings were contracted with vasoconstrictors, and the developed tension changes were considered control values. The rings were then pre- treated with $30{\mu}M$ nitroglycerin, nicardipine, verapamil, and papaverine, respectively, for 40 minutes and rinsed with the physiologic buffered salt solution three times every 15 min. The vasoconstrictor-induced tension changes after the previous procedure were considered experimental values. Data are expressed as the percentage tension induced by vasoconstrictors before and after pretreatment with vasodilators. Result: Nicardipine depressed vasoconstriction induced by norepinephrine, angiotensin II (All), and U46619 in both the BA and the CA more significantly than did nitroglycerin (p<0.01) and verapamil (p<0.05). Verapamil depressed vasoconstriction induced by 5-hydroxytryptamine (5HT), All, and U46619 in the BA and by 5HT in the CA more significantly than did nitroglycerin (p<0.01). Conclusion: These findings suggest that both nicardipine and verapamil effectively depressed vasoconstrictor action. Nicardipine is thought to be more effective than verapamil for the prevention of vasoconstrictor action.
Keywords
Nitroglycerin; Nicardipine; Verapamil; Papaverine; Vascular tone;
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