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Alteration of Apurinic/Apyrimidinic Endonuclease-1/Redox Factor-1 in Human Mon-small Cell Lung Cancer  

Yoo, Dae-Goon (Department of Physiology, College of Medicine, Chungnam National University)
Song, Yun-Jeong (Department of Physiology, College of Medicine, Chungnam National University)
Cho, Eun-Jung (Department of Physiology, College of Medicine, Chungnam National University)
Kang, Min-Woong (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Chungnam National University)
Han, Jong-Hee (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Chungnam National University)
Na, Myung-Hoon (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Chungnam National University)
Lim, Seung-Pyung (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Chungnam National University)
Yu, Jae-Hyeon (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Chungnam National University)
Jeon, Byeong-Hwa (Department of Physiology, College of Medicine, Chungnam National University)
Lee, Young (Department of Thoracic and Cardiovascular Surgery, College of Medicine, Chungnam National University)
Publication Information
Journal of Chest Surgery / v.40, no.8, 2007 , pp. 529-535 More about this Journal
Abstract
Background: An imbalance between oxidants and antioxidants leads to oxidative stress, and this has been proposed to play an important role in the pathogenesis of lung neoplasm. Apurinic/apyrimidinic endonuclease-1/redox factor-1 (APE/ref-1) is a multifunctional protein involved in DNA base excision repair and the redox regulation of many transcription factors. However, the alteration of the expressed levels of APE/ref-1 in non-small cell lung cancer is unknown. Material and Method: Forty-nine patients with surgically resected non-small cell lung cancer (NSCLC) were included in this study. Immunohistochemical staining with APE/ref-1 antibodies was performed, and their expressions were analyzed via Western blotting for specific antibodies. Result: APE/ref-1 was localized at the nucleus and mainly in the non-tumor region of the NSCLC tissue specimens; it was expressed in the cytoplasm and nucleus of the NSCLC. The nuclear and cytoplasmic expressions of APE/ref-1 in lung cancers were markedly up-regulated in the NSCLC, and this was correlated with the clinical stage. Catalase, as first-line antioxidant defense, was dramatically decreased in the NSCLC. Conclusion: Taken together, our results suggest that APE/ref-1, and especially cytoplasmic APE/ref-1, was upregulated in the lung cancer regions, and this may contribute to the compensatory defense system against oxidative stress. A low expression of catalase might have fundamental effects on the extracellular redox state of lung tumors, along with the potential consequences for the tumors.
Keywords
Lung neoplasms; Proteins;
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