Browse > Article

Changes of Serum IgM, IgG in Pig's Xenograft Perfusion and Immunofluorescence Changes of the Deposition of IgM, IgG in the Xenograft in Dogs  

Lee, Doo-Yun (Department of Thoracic and Cardiovascular Surgery, Yongdong Severance Hospital, Institute of Chest Disease, Yonsei University College of Medicine)
Paik, Hyo-Chae (Department of Thoracic and Cardiovascular Surgery, Yongdong Severance Hospital, Institute of Chest Disease, Yonsei University College of Medicine)
Jeon, Se-Eun (Department of Thoracic and Cardiovascular Surgery, Yongdong Severance Hospital, Institute of Chest Disease, Yonsei University College of Medicine)
Kim, Eun-Young (Department of Thoracic and Cardiovascular Surgery, Yongdong Severance Hospital, Institute of Chest Disease, Yonsei University College of Medicine)
Nam, Jin-Young (Department of Diagnostic Pathology, Yongdong Severance Hospital, Yonsei University College of Medicine)
Hong, Soon-Won (Department of Diagnostic Pathology, Yongdong Severance Hospital, Yonsei University College of Medicine)
Hwang, Jung-Joo (Department of Thoracic and Cardiovascular Surgery, Eulji University Hospital, Eulji University)
Wada, Hiromi (Department of Thoracic and Cardiovascular Surgery, Graduate School of Medicine, Kyoto University)
Bando, Toru (Department of Thoracic and Cardiovascular Surgery, Graduate School of Medicine, Kyoto University)
Publication Information
Journal of Chest Surgery / v.40, no.7, 2007 , pp. 467-472 More about this Journal
Abstract
Background: Lung transplantation is the definitive therapy for end stage lung disorders. The success of allogenic lung transplantation has led to an increasing shortage of donor lungs from humans, including cadavers, and attention has now turned to transplantation of lungs from other species. However, there are many biological hurdles when using organs from other species because of hyperacute rejection after discordant xenotransplantation. Material and Method: Pigs (n=6, weighing $20{\sim}30kg$ each) for the donors and mongrel dogs (n=6, weighing $20{\sim}28kg$ each) for the recipients were used in this experiment. The left kidney of a pig was perfused to a mongrel dog for 30 minutes through the femoral artery and vein of the dog, and the right kidney was perfused for 30 minutes sequentially. Then, both lungs of the pig were perfused to the dog through the pulmonary artery and left atrium with using the same time intervals. The levels of IgM and IgG were measured from the blood and specimens of the kidney and lung. Result: The average levels of serum IgM gradually decreased after the perfusion, but the average levels of serum IgG did not charge from before to after perfusion. The immunohistochemical findings revealed decreased deposition of IgG and IgM after the perfusion. Conclusion: We conclude that the levels of the serum natural antibodies would be decreased with pre-transplantation xenograft perfusion in the recipient and the occurrence rate of hyperacute rejection after transplantation would be decreased.
Keywords
Lung transplantation; Xenotransplantation; Rejection;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Juvenelle AA, Citred C, Wles CE, Steard JD. Pneumonectomy with replantation of the lung in dog for physiologic study. J Thorac Surg 1951;21:111-5   PUBMED
2 Auchincloss H Jr, Sachs DH. Xenogenic transplantation. Annu Rev Immunol 1988;16:433-70   DOI   ScienceOn
3 Macchiarini P, Oriol R, Dartevelle P, et al. Evidence of human non $\alpha$-galactosyl antibodies involved in the hyperacute rejection of pig lungs and their removal by pig organ perfusion. J Thorac Cardiovasc Surg 1998;11:831-43
4 Cantu E, Parker W, Platt JL, Davis RD. Pulmonary xenotransplantation: rapidly progressing into the unknown. Am J Transplant 2004;4:25-35   PUBMED
5 Lin SS, Weldner BC, Platt JL, et al. The role of antibodies in acute vascular rejection of pig-to-baboon cardiac transplants. J Clin Invest 1998;101:1745-56   DOI   ScienceOn
6 Brenner P, Reichenspurner H, Reichart B, et al. Prevention of hyperacute xenograft rejection in orthotopic xenotransplantation of pig hearts into baboons using immunoadsorption of antibiotics and complement factors. Transplantation 2000;13:508-17   DOI   ScienceOn
7 Demikhov VP. Transplantation of vital organs in experiments. Moscow: Medgiz. 1960
8 Fischel RJ, Matas AJ, Bolman RM, et al. Cardiac xenografting in the pig-to-rhesus monkey model. Manipulation of anti- endothelial antibody prolongs survival. J Heart Lung Transplant 1992;11:965-73   PUBMED
9 Takahashi M, Nakajima S, Kato S, et al. Role of xenoreactive natural antibodies in pig-to-human lung xenotransplantation. Transplant Proc 2002;34:2739-44   DOI   ScienceOn
10 Nakajima F, Nakajima S, Kato H, et al. Analysis of hyperacute rejection in new born pig-to-dog lung transplantation. Transplant Proc 2002;32:1131-40
11 Evans RW, Orians CE, Asher NL. The potential supply of organ donors. An assessment of the efficiency of organ procurement efforts in the united states. JAMA 1992;267:239-46   DOI
12 Hardy JD, Chavez KM, Kurrus FD, et al. Heart transplantation in man. Developmental studies and report of a case. JAMA 1964;188:1132-40   DOI   PUBMED   ScienceOn
13 Derom F, Barbier F, Ringoir S, et al. Ten-month survival after lung homotransplantations in man. J Thorac Cardiovasc Surg 1971;61:835-46   PUBMED
14 Byrne GW, McCurry KR, Martin MJ, et al. Transgenic pigs expressing human CD59 and decay-accelerating factor produce an intrinsic barrier to complement-mediated damage. Transplantation 1997;63:149-55   DOI   ScienceOn
15 Platt JL, Vercelotti GM, Dalmasso AP. Transplantation of discordant xenografts. A review of progress. Immunol Today 1990;11:450-6   DOI   ScienceOn