Browse > Article

Nuclear Medicine Methods of Rejection Diagnosis in Transplanted Rat Model  

Chung, Won-Sang (Dept. of Thoracic and Cardiovascular Surgery, College of Medicine, Hanyang University)
Kim, Sang-Heon (Dept. of Thoracic and Cardiovascular Surgery, College of Medicine, Hanyang University)
Kim, Hyuck (Dept. of Thoracic and Cardiovascular Surgery, College of Medicine, Hanyang University)
Kim, Young-Hak (Dept. of Thoracic and Cardiovascular Surgery, College of Medicine, Hanyang University)
Kang, Jung-Ho (Dept. of Thoracic and Cardiovascular Surgery, College of Medicine, Hanyang University)
Choi, Yun-Young (Dept. of Nuclear Medicine, College of Medicine, Hanyang University)
Lee, Chul-Beom (Dept. of Thoracic and Cardiovascular Surgery, College of Medicine, Hanyang University, Guri Hospital)
Publication Information
Journal of Chest Surgery / v.36, no.6, 2003 , pp. 379-383 More about this Journal
Abstract
Background: An accurate diagnosis of the severity of the rejection after a heart transplantation relies on endomyo-cardial biopsy, but because of its invasiveness and the need for repeated examination makes it is an inappropriate monitoring method. Therefore, we have tried to find a monitoring method that is continuous and less invasive. Material and Method: Heterotopic heart transplantation using Ono-Lindsey Method was done in 20 rats, and then $^{99m}$ Tc-Pyrophosphate (PYP) scan was done after a month, Uptake ratio of transplanted heart to vertebrae (H/V) was obtained to be compared with the biopsy result. Result: Rejection was defined when the H/V uptake ratio was higher than 0.09, and we compared the uptake ratio with the results of biopsy. The result was true positives was 3, true negatives 12, false negatives 2, andfalse positives 3. Therefore sensitivity was 60% and specificity was 80%, diagnostic value was 75%. Conclusion: $^{99m}$Tc-Pyrophosphate (PYP) scan was a useful method for the evaluation of the heart transplantation rejection and it will be helpful for monitoring rejection as an non-invasive and simple method.hod.
Keywords
Heart transplantation; Graft rejection; Rejection; Radionuclide imaging;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ono K, Lindsey ES. Improved technique of heart transplant in Rats. J Thorac Cardiovasc Surg 1969;57:225-9   PUBMED
2 Caves PK, Stison EB, Billingham ME, Shumway NE. Serial transvenous biopsy of the transplanted human heart. Improved management of acute rejection episodes. Lancet 1974;1:821-6   PUBMED
3 Habert R, Weber M, Reichenspurner H, et al. Frequency analysis of the surface electrocardiogram for recognition of acute rejection after orthotopic cardiac transplantation in man. Circulation 1987;76:101-8   DOI
4 Schuetz A, Kemkes BM, Breuer M, et al. Kinetics and dynamics of acute rejection after heterotopic heart transplantation. J Heart Transplant 1992;11:147-53.
5 Bergland J, Hwang K, Drescoll R, et al. Corobary blood flow & Thallium 201 uptake in rejecting rat heart transplantation. J Heart transplant 1989;8:147-53.
6 Yamamoto S, Bergland J, Michlek SM, et al. Uptake of myocardial Imaging agents by rejecting & non-rejecting cardiac transplants: A comparative clinical study of Thallium 201, Technetium 99m & Gallium 67. J Nucl Med 1989;30: 1464-9   PUBMED
7 Mckillop JH, McDougall R, Goris MC, Mason JW, Reitz BA. Failure to diagnose cardiac transplant rejection with Tc-99m PYP images. Clin Nucl Med 1981;6:373-7
8 Schroeder JS, Popp RC, Stinson EB, et al. Acute rejection following cardiac transplantation: Phonocardiographic and ultrasonic observations. Circulation 1969;40:155-64   DOI   PUBMED   ScienceOn
9 Bergland J, Carr EA, Carol M, et al. Uptake of myocardial imaging agents by rejected heart. J Heart Transplant 1985; 4:536-40
10 Walpoth BH, Tschopp A, Lazeyras F, et al. Magnetic resonance spectroscopy for assessing myocardial rejection in the transplanted rat heart. J Heart Transplant 1993;12: 271-82
11 Menequetti JC, Carmargo EE, Soares J, et al. Gallium 67 imaging in human heart transplantation: Correlation with endomyocardial biopsy. J Heart Transplant 1987;6:171-6
12 Novitzky D, Boniaszczuk J, Cooper D, et al. Prediction of acute cardiac rejection using radionucleide technique. S Afr Med J 1984;56:5-7
13 Silver MA, Grusk BB, Dillehay GL. Gallium-67 scanning is not useful in detecting cardiac allograft rejection. J Heart Transplant 1988;7:65