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http://dx.doi.org/10.15429/jkomor.2022.22.2.102

Anti-Obesity Effects and the Regulation of Energy Metabolism in Skeletal Muscle Tissues of Allii Fistulosi Bulbus Extract in High Fat Diet-Induced Obesity Mice  

Yoon Yong Choi (College of Korean Medicine, Dongguk University)
Hyeon Soo Lee (College of Korean Medicine, Dongguk University)
Su Yeon Baik (College of Korean Medicine, Dongguk University)
Sumin Lim (College of Korean Medicine, Dongguk University)
Hyo Won Jung (College of Korean Medicine, Dongguk University)
Seok Yong Kang (Korean Medicine R&D Institute, Dongguk University)
Yong-Ki Park (College of Korean Medicine, Dongguk University)
Publication Information
Journal of Korean Medicine for Obesity Research / v.22, no.2, 2022 , pp. 102-114 More about this Journal
Abstract
Objectives: We investigated the effects of Allii Fistulosi Bulbus (AFB) on high fat diet (HFD)-induced obesity in mice and the regulation of energy metabolism in muscle tissues of mice. Methods: The C57BL/6 mice (6 weeks, male) were fed a HFD for 8 weeks and then administrated with AFB extract at 500 mg/kg (p.o.) once daily for 4 weeks. The body weight (BW), muscle weight, calorie intake, fasting blood glucose (FBG) and serum glucose, insulin, and low-density lipoprotein-cholesterol (LDL-C) levels were measured in mice. It was also observed the histological changes of pancreas, liver, and fat tissues with hematoxylin and eosin staining. It was investigated the phosphorylation of insulin receptor substrate 1 (IRS-1), Ser/Thr kinase (AKT), and adenosine monophosphate-activated protein kinase (AMPK), and the expression of phosphoinositide 3-kinase, glucose transporter type 4 (GLUT4), and sirtuin1 (Sirt1) in gastrocnemius tissues by western blot, respectively. Results: The increases of BWs, calorie intakes and FBG levels in obesity mice were decreased significantly by the administration of AFB extract. The AFB extract administration was reduced significantly serum levels of glucose, insulin, and LDL-C in obesity mice. The AFB extract inhibited lipid accumulation in liver tissues, hyperplasia of pancreatic islets, and enlargement of fat tissues in obesity mice. The phosphorylation of IRS-1 and AKT was increased significantly in muscle tissues and AMPK phosphorylation and the GLUT4 and Sirt1 expression were decreased significantly in muscle tissues after the AFB administration. Conclusions: Our study indicates that AFB extract improves symptoms of obesity through regulation of energy regulating proteins in muscle tissues.
Keywords
Obesity; High-fat diet; Allii Fistulosi Bulbus; Energy metabolism;
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Times Cited By KSCI : 5  (Citation Analysis)
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