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http://dx.doi.org/10.15429/jkomor.2018.18.2.64

Effect of Platycodon grandiflorum Fermentation with Salt on Fermentation Characteristics, Microbial Change and Anti-obesity Activity  

Shin, Na Rae (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University)
Lim, Sokyoung (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University)
Kim, Hojun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University)
Publication Information
Journal of Korean Medicine for Obesity Research / v.18, no.2, 2018 , pp. 64-73 More about this Journal
Abstract
Objectives: This study investigated the effect on microbial ecology, fermentation characteristics and anti-obesity of Platycodon grandiflorum (PG) fermentation with salt. Methods: PG was fermented for four weeks with 2.5% salt and the characteristics of fermented PG were performed by measuring pH, total sugar content, viable bacteria number and microbial profiling. Also, we measured total polyphenol, flavonoid and the percent of inhibition of lipase activity and lipid accumulation. Results: Salt added to PG for fermentation had an effect on pH, total sugar, total and the number of lactic acid bacteria. Total sugar and pH were reduced and number of total and lactic acid bacteria were increased after fermentation. The majority of bacteria for fermentation were Lactobacillus plantarum, Leuconostoc psedomesenteroides and Lactococcus lactis subspecies lactis regardless of salt addition. However, microbial compositions were altered by added salt and additional bacteria including Weissella koreensis, W. viridescens, Lactobacillus sakei and Lactobacillus cuvatus were found in fermented PG with salt. Total flavonoid was increased in fermented PG and lipid accumulation on HepG2 cells treated with fermented PG was reduced regardless of salt addition. Moreover, fermented PG without salt suppressed lipase activity. Conclusions: Addition of salt for PG fermentation had influence on fermentation characteristics including pH and sugar content as well as number of bacteria and microbial composition. In addition, fermented PG showed anti-obesity effect by increasing flavonoid content and inhibition of lipase activity and lipid accumulation.
Keywords
Platycodon grandiflorum; Fermentation; Salts; Lactic acid bacteria; Anti-obesity agents;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Hong MW. Statistical analyses of Platycodi radix prescriptions. Korean Journal of Pharmacognosy. 1974 ; 5(1) : 61-7.
2 Jang YE, Seo BI. The anti-obesity effects of Platycodi Radix, combination of Platycodi Radix and Cyperi Rhizoma on obesity induced by high fat diet. Kor J Herbology. 2016 ; 31(3) : 240 -1.
3 Byun BH. Antiobesity effects of Platycodon grandiflorum extract on body weight changes and serum lipid profiles of obese rats induced high fat diet. Korean J Life Sci. 2003 ; 13(6) : 896-902.   DOI
4 Park KY. Increased health functionality of fermented foods. Food Industry and Nutrition. 2012 ; 17(1) : 1-8.
5 Son CY, Song BJ, Ma JY, Kwon KI. Anti-platelet aggregation study of fermented Galgeun Tang and fermented Ssanghwa Tang. Yakhak Hoeji. 2011 ; 55(5) : 374-8.
6 Choi W, Song J, Park MH, Yu HJ, Park H. Effect of fermented Platycodon grandiflorum extract on cell proliferation and migration in bovine aortic endothelial cells. J Life Sci. 2016 ; 26(1) : 59-67.   DOI
7 Kang DH, Kim JS. Functionality analysis of Korean medicine fermented by lactobacillus strains. J Microbiol Biotechnol. 2011 ; 39(3) : 259-65.
8 Choi YK, Sul JU, Park SK, Yu SN, Kim SH, Rhee MS, et al. Research trends of fermented medicinal herbs - based on their clinical efficacy and safety assessment. J Life Sci. 2012 ; 22(12) : 1729-39.   DOI
9 Kang YH, Kim KK, Kim TW, Yang CS, Choe M. Evaluation of the anti-obesity activity of Platycodon grandiflorum root and Curcuma longa root fermented with Aspergillus oryzae. Korean J Food Sci Technol. 2015 ; 47(1) : 111-8.   DOI
10 Lee KS, Kim JN, Chung HC. Study on anti-oxidative activities and beverage preferences relating to fermented lotus root and Platycodon grandiflorum extracts with sugar through lactic acid fermentation. The East Asian Society of Dietary Life. 2015 ; 25(1) : 183-92.   DOI
11 Park SJ, Park KY, Jun HK. Effects of commercial salts on the growth of kimchi-related microorganisms. J Korean Soc Food Sci Nutr. 2001 ; 30(5) : 806-19.
12 Shin NR, Wang JH, Lim D, Lee MJ, Lim H. Microbial change and fermentation characteristics during Samjung-Hwan natural fermentation. J Korean Med Obes Res. 2015 ; 15(2) : 123-30.   DOI
13 Han KI, Kim MJ, Kwon HJ, Kim YH, Kim WJ, Han MD. The effect of container typees on the growth of bacteria during Kimchi fermentation. The Korean Journal of Food And Nutrition. 2013 ; 26(2) : 249-57.   DOI
14 Lee SY, Yoo KM, Moon BK, Hwang IK. A study on the development of vinegar beverage using Yacon Roots (Smallanthus sonchifolius) and analysis of components changes during the fermentation. Korean J Food Cook Sci. 2010 ; 2(1) : 95-103.
15 Jung JY, Lee SH, Jin HM, Hahn Y, Madsen EL, Jeon CO. Metatranscriptomic analysis of lactic acid bacterial gene expression during kimchi fermentation. Int J Food Microbiol. 2013 ; 163(2) : 171-9.   DOI
16 Baek SY, Kim JS, Mun JY, Lee CH, Park YK, Yeo SH. Quality characteristics of detoxified Rhus verniciflua vinegar fermented using different acetic acid bacteria. Korean J. Food Preserv. 2016 ; 23(3) : 347-54.   DOI
17 Kim YJ, Cho HJ, Yu SN, Kim KY. Diversity of marine microbes by PRC-DGGE. Kor J Fish Aquat Sci. 2007 ; 40(6) : 356-61.   DOI
18 Caiola MG, Billi D, Friedmann EI. Effect of desiccation on envelopes of the cyanobacterium Chroococcidiopsis sp. (Chroococcales). Eur J Phycol. 1996 ; 31(1) : 97-105.   DOI
19 Ehrhardt DW, Wais R, Wais SR. Calcium spiking in plant root hairs responding to Rhizobium nodulation signals. Cell. 1996 ; 85(5) : 673-754.   DOI
20 Cha SK. Probiotic microorganisms and lactic acid bacterial foods. J Microbiol. 2000 ; 26(2) : 13-21.
21 Rodriguez H, Curiel JA, Kandete JM, Rivas B, Felipe FL, Gomez-Cordoves, et al. Food phenolics and lactic acid bacteria. Int J Food Microbiol. 2009 ; 132(2) : 79-90.   DOI
22 Back G, Goo BG, Ahn BJ, Park JK. Effects of water-soluble polysaccharides from tott on lipid absorption and animal body weight. J Korean Soc Food Sci Nutr. 2013 ; 42(4) : 556-62.   DOI