Browse > Article
http://dx.doi.org/10.15429/jkomor.2016.16.1.11

Microbiological Characteristics and Cytoprotective Effects of Samjung-Hwan Fermented by Lactic Acid Bacteria  

Chang, Seju (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University)
Wang, Jing-Hua (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University)
Shin, Na Rae (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University)
Kim, Hojun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Dongguk University)
Publication Information
Journal of Korean Medicine for Obesity Research / v.16, no.1, 2016 , pp. 11-18 More about this Journal
Abstract
Objectives: To confirm microbiological change and cytoprotective effect of Samjung-hwan (SJH) which fermented by Lactic acid bacteria from natural fermented SJH. Methods: SJH was fermented by Lactobacillus brevis and Lactococcus lactis subsp. lactis from natural fermented SJH. After 1 week of fermentation, we analysed pH and microbial profiling. We also performed measuring total polyphenol total flavonoid contents and 1,1-Diphenyl-2-picryhydrazyl (DPPH) free radical scavenging activity to investigate antioxidant ability. Cell viability was performed by using HepG2 cell. Results: pH of lactic acid bacteria inoculated group and non-inoculated group was decreased and total counts of lactic acid bateria for both group was increased after fermentation of SJH. Total polyphenol and flavonoid contents and DPPH free radical scavenging activity was increased in both group. Total polyphenol contents of lactic acid bacteria Inoculated group is more increased than non-inoculated group. HepG2 cell viability was increased in both group. Conclusions: SJH fermentd by Lactobacillus brevis and Lactococcus lactis subsp. lactis shows change in microbiological character and has cytoprotective effect. Further studies are required for investigating function of lactic acid bacteria during fermentation of SJH.
Keywords
Samjung-hwan; Fermentation; Lactobacillus; Cytoprotection; Antioxidants;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Kim HG, Bose S, Kim DI, Koo BS, Kim HJ. Effect of fermented Lotus extract on glucose intolerance and lipid metabolism-related gene expression. J Korean Med Rehab. 2014 ; 24(1) : 1-12.
2 An YT. Health functional food and probiotic. The Korean Society of Food and Nutrition Conference, Busan, Korea. Uijeongbu : The Korean Society of Food and Nutrition. 2011 : 32-43.
3 Shin NR, Wang JH, Lim DW, Lee MJ, Kim HJ. Microbial change and fermentation charactristic during samjung-hwan natural fermentation. J Korean Med Obes Res. 2015 ; 15(2) : 123-30.   DOI
4 Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat Protoc. 2007 ; 2(4) : 875-7.   DOI
5 Kim KY, Song HJ. Herbal processology. Seoul : Shinil Press. 2002 : 547.
6 Shin YJ, Kim HJ, Lee MY. The effect of fermentated Ephedra sinica on obese rats fed by high fat diet. J Orient Rehab Med. 2009 ; 19(4) : 37-57.
7 Kang DH, Kim JS. Functionality analysis of Korean medicine fermented by lactobacillus strains. J Metabol Bariatr Surg. 2011 ; 39(3) : 259-65.
8 Rodriguez H, Curiel JA, Kandete JM, Rivas B, Felipe FL, Gomez-Cordoves, et al. Food phenolics and lactic acid bacteria. Int J Food Microbiol. 2009 ; 132(2) : 79-90.   DOI
9 Prakash D, Suri S, Upadhyay G, Singh BN. Total phenol, antioxidant and free radical scavenging activities of some medicinal plants. Int J Food Sci Nutr. 2007 ; 58(1) : 18-28.   DOI
10 Jeong HJ, Kim SY, Jeong JC. Effect of Samjunghwan on obesity and lipid metabolism in high fat diet rats. J Korean Orient Med. 2006 ; 27(3) : 24-35.
11 Kim JH, Kim GW, Koo BS. Effect of Samjung-hwan (sanjingwan) on obesity and lipid metabolism in rats with high fat diet. J Orient Neuropsychiatry. 2009 ; 20(2) : 47-60.
12 Kim AJ, Park SJ, Rho JO. Mulberry fruit extract consumption is inversely associated with hyperlipidemia in middle-aged men. Korean J Food Nutr. 2008 ; 21(2) : 121-6.
13 Kim YC, Jun M, Jeong WS, Chung SK. Antioxidant properties of flavone C-glycosides from atractylodes japonica leaves in human low-density lipoprotein oxidation. J Food Sci. 2005 ; 70(9) : S575-80.
14 Sung NK, Kim SH, Seo YB, Oh AH. Effect on blood glucose, hypertension of Lycium chinense miller. J Herbol. 1994 ; 9 : 161-71.
15 Song M, Bose S, Kim H. Anti-obesity effects of fermented Samjung-hwan in high fat diet rats. J Korean Med Obes Res. 2013 ; 13(1) : 17-23.
16 Wang JH, Bose S, Kim HG, Han KS, Kim HJ. Fermented Rhizoma atractylodes macrocephalae alleviates high fat diet induced obesity in association with regulation of intestinal permeability and microbiota in rats. Sci Rep. 2015 ; 16(5) : 83-91.
17 Park JH, Kim HJ, Lee MJ. The role of gut microbiota in obesity and utilization of fermented herbal extracts. J Korean Med Obes Res. 2009 ; 9(1) : 1-14.
18 Wang JH, Bose S, Kim GC, Hong SU, Kim JH, Kim HJ. Flos Lonicera ameliorates obesity and associated endotoxemia in rats through modulation of gut permeability and intestinal microbiota. PLos One. 2014 ; 9(1) : e86117.   DOI