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Effects of Fermented Lotus Extracts on the Differentiation in 3T3-L1 Preadipocytes  

Lee, Sin Ji (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University)
Bose, Shambhunath (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University)
Lee, Su-Jin (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University)
Jeong, Ji-Eun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University)
Koo, Byung-Soo (Department of Neuropsychiatry, College of Oriental Medicine, Dongguk University)
Kim, Dong-Il (Department of Obstetrics and Gynecology, College of Oriental Medicine, Dongguk University)
Kim, Hojun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University)
Publication Information
Journal of Korean Medicine for Obesity Research / v.13, no.2, 2013 , pp. 74-83 More about this Journal
Abstract
Objectives: This study was performed to evaluate the effects of fermented lotus extracts on the inhibition of differentiation in 3T3-L1 preadipocytes. Methods: Extracts of lotus leaf and lotus root were fermented using 4 different probiotics separately, including Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium breve, and Bifidobacterium longum. Inhibition of preadipocyte differentiation was examined by Oil red O dye staining. Expressions of adipogenic transcription factors including CCAAT/enhancer binding proteins (C/$EBP{\alpha}$) and peroxisome proliferators-activated receptor ${\gamma}$ ($PPAR{\gamma}$) were analyzed by real time polymerase chain reaction and Western blotting analysis. Results: Fermented lotus extracts inhibited adipogenic transcription factors by inhibiting preadipocytes differentiation. All of the groups fermented by 4 kinds of probiotics showed reduction in Oil Red O dye staining. Bifidobacterium breve showed the most effective inhibition of C/$EBP{\alpha}$. Bifidobacterium breve and Bifidobacterium longum showed the best downregulation of $PPAR{\gamma}$ expressions compared with the control and the unfermented lotus group. Conclusions: Fermented lotus extracts showed significant effects on inhibition of preadipocyte differentiation in 3T3-L1 preadipocytes showing correlation with insulin sensitivity and lipid metabolism related with obesity.
Keywords
3T3-L1 preadipocyte; Lotus; Probiotics; Fermentation; Obesity; Insulin resistance;
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