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http://dx.doi.org/10.14374/HFS.2022.30.4.259

Erythrocyte Micronucleus Test of Pomace Schisandra chinensis Extracts Using ICR Mouse  

Seokho, Kim (QBM Co. Ltd.)
Sun Youn, Kim (Croen Inc.)
Young-Suk, Kim (Glucan Co. Ltd.)
Jong-Min, Lim (Glucan Co. Ltd.)
Bon-Hwa, Ku (Glucan Co. Ltd.)
Kyeong Tae, Kwak (QBM Co. Ltd.)
Byeong Yeob, Jeon (QBM Co. Ltd.)
Publication Information
Herbal Formula Science / v.30, no.4, 2022 , pp. 259-267 More about this Journal
Abstract
Objectives : In this study, erythrocyte micronucleus test of pomace Schisandra chinensis extracts was conducted in order to up-cycling to a high value-added industry using by-products discarded in the production process of Schisandra chinensis products and active ingredients such as dibenzocyclooctadiene lignans in Schisandra chinensis. Methods : The micronucleus test was performed according to the 'OECD Guidelines'. Including the negative control group(0 mg/kg) and the positive control group(CPA 70 mg/kg), pomace Schisandra chinensis extracts were orally administered to ICR mouse at doses of 500, 1,000, and 2,000 mg/kg. After sacrificing the experimental animals bone marrow cells were collected and micronucleated polychromatic erythrocyte were counted. And genetic toxicity was confirmed according to the frequency of micronucleus. Results : As a result of the micronucleus test, there were no changes in body weight, clinical signs, or death in any group. But, a significant increase was observed in the frequency of micronucleated polychromatic erythrocyte among polychromatic erythrocytes in the positive control group administered with CPA compared to the negative control group(p<0.05). Whereas, no significant increase was observed in the group administered with pomace Schisandra chinensis extracts compared to the negative control group. Conclusions : Pomace Schisandra chinensis extracts did not induce micronucleus in bone marrow cells of ICR mouse up to a concentration of 2,000 mg/kg, and it was judged that no genetic toxicity was observed.
Keywords
Pomace Schisandra chinensis; up-cycling; genetic toxicity; erythrocyte micronucleus test; OECD;
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Times Cited By KSCI : 8  (Citation Analysis)
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