Browse > Article
http://dx.doi.org/10.14374/HFS.2021.29.4.191

Effect of Ullmus macrocarpa Hance Ethanol extract (Ulmus) on Improvement of allergic responses in RBL-2H3 mast Cells  

Do, Hyun Ju (New Drug Development Center, Daegu-Gyeongbuk Medical Innovation Foundation)
Oh, Tae Woo (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM))
Publication Information
Herbal Formula Science / v.29, no.4, 2021 , pp. 191-203 More about this Journal
Abstract
Objectives : In this study, we investigate the anti-allergic effects of Ullmus macrocarpa Hance (Ulmus) on RBL-2H3 mast cell (basophilic leukemia cell line), which are mediated by FcεRIs. Methods : We evaluated the effect of the ethanol extract of Ulmus on the allergic inflammatory response in IgE-antigen-mediated RBL-2H3 cells. Cell toxicity was determined by MTT assay and the markers of degranulation such as beta-hexosaminidase, histamine, PGD2, TNF-α, IL-4, IL-6 production of inflammatory mediators and FcεRI-mediated protein expression by western blot. Results : Ulmus inhibited degranulation and production of allergic mediators (e.g., TNF-α, IL-4, and IL-6) in them. Ulmus reduced histamine levels, expression of FcεRI signaling-related genes such as Lyn, Syk, and Fyn, and extracellular signal-regulated kinase phosphorylation in mast cells. Also, Ulmus reduced PGD2 release and cyclooxygenase-2 expression, and cytosolic phospholipase A2 phosphorylation in FcεRI-mediated RBL-2H3 mast cells. Conclusions : These results indicate that Ulmus exhibits anti-allergic activity through inhibition of degranulation and inflammatory mediators and cytokine release. These findings suggest that Ulmus may have potential as a prophylactic and therapeutic agent for the treatment of various allergic diseases.
Keywords
Ullmus macrocarpa Hance; RBL-2H3 mast cell; anti-allergic effect; inflammation mediators;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Dykewicz MS, Fineman S, NicklasR, Lee R, Blessing-Moore J, Li JT. Executive summary of joint task force practice parameters on diagnosis and management of rhinitis. Ann Allergy Asthma Immunol. 1998;81:463-468.   DOI
2 Korean Journal of Asthma, Allergy and Clinical Immunology. Asthma and Allergy disease. Seoul: koonja publisher. 2002;66:431-433.
3 Galli SJ. New concepts about the mast cell. N Engl J Med. 1993; 328(4): 257-265.   DOI
4 KM Ahn. Role of mast cell in allergic inflammation and innate immunity. Korean Journal of Pediatrics. 2004;47:11-15.
5 Poole JA, Rosenwasser LJ. The role of immunoglobulin E and immune inflammation : implications in allergic rhinitis. Curr Allergy Asthma Rep. 2005;5(3):252-258.   DOI
6 Lin K, Huang D, Huang D, Tzeng S and Lin W. Inhibition of ampk through lyn-syk-akt enhances fcεri signal pathways for allergic response. J Mol Med. 2016;94:183-194.   DOI
7 Ahn DK, Nac HS. Oriental medicine and immunology. Paju:openbooks. 1994:15-48.
8 Lee SJ, Lim KT. Glycoprotein isolated from Ulmus davidiana Nakai regulates expression of iNOS and COX-2 in vivo and in vitro. Food Chem Toxicol. 2007;45:990-1000.   DOI
9 Kim KS, Lee SD, Kim KH, Kil SY, Chung KH, Kim CH. Suppressive effects of a water extract of Ulmus davidiana Planch (Ulmaceae) on collageninduced arthritis in mice. J Ethnopharmacol. 2005;97:65-71.   DOI
10 Kim JP, Kim WG, Koshino H, Jung J, Yoo ID. Sesquiterpene O-naphthoquinones from the root bark of Ulmus davidiana. Phytochemistry. 1996;43: 425-430.   DOI
11 Zheng MS, Li G, Li Y, Seo CS, Lee YK, Jung JS, Song DK, Bae HB, Kwak SH, Chang HW, Kim JR, Son JK. Protective constituents against sepsis in mice from the root barks of Ulmus davidiana var. japonica. Arch Pharm Res. 2011; 34:1443-1450.   DOI
12 Busse WWW, Lemanske RF Jr. Asthma. N Engl J. Med. 2001;44(5):350-62.
13 Woo HS, Kim CH. The Review of Oriental Medical Therapy on Allergic Rhinitis. J Korean Oriental Med.2006;27(1):155-64.
14 Kay AB. Allergy and allergic diseases. First of two parts. N Engl J Med 2001; 344(1): 30-37.   DOI
15 Lee E, Chol EJ, Cheong H, Kim YR, Ryu SY, Kim KM. Anti-allergic actions of the leaves of castanea crenata and isolation of an active component responsible for the inhibition of mast cell degranulation. Archives of Pharmacal Research. 1999;22:320-323.   DOI
16 Choi SY, Lee S, Choi WH, Lee Y, Jo YO, Ha TY. Isolationand anti-inflammatory activity of Bakuchiolfrom Ulmus davidiana var. japonica. J Med Food. 2010;13:1019-1023.   DOI
17 Beaven MA, Rogers J, Moore JP, Hesketh TR, Smith GA, Metcalfe JC. The mechanism of the calcium signal and correlation with histamine release in 2H3 cells. J. Biol. Chem. 1984;259: 7129-7136.   DOI
18 Mekori YA, Metcalfe DD. Mast cells in innate immunity. Immunol Rev. 2000;173: 131-140.   DOI
19 Toru H, Pawankar R, Ra C, Yata J, Nakahata T. Human mast cells produce IL-13 by high-affinity IgE receptor cross-linking:enhanced IL-13 production by IL-4-primed human mast cells. J Allergy Clin Immunol. 1998;102:491-502.   DOI
20 Murphy, R.C., Gijon, M.A. Biosynthesis and metabolism of leukotrienes. Biochem J. 2007; 405(3):379-395.   DOI
21 Moon SH, Jang HJ, Park YS, Lee WY, Lim DH, Kim JH. Fractional exhaled nitric oxide in Korean children with allergic rhinitis. Allergy Asthma Respir Dis. 2015;3(6):439-445.   DOI
22 Gurish MF, Austen KF. The diverse roles of mast cells. J Exp Med 2001; 194(1): F1-5.   DOI
23 Salamon P, Shoham NG, Gavrieli R, Wolach B, Mekori YA. Human mast cells release interleukin-8 and induce neutrophilchemotaxis on contact with activated T cells. Allergy. 2005;60:1316-1319.   DOI
24 Fukui, H. Progress in Allergy Signal Research on Mast Cells: Up-Regulation of Histamine Signal-Related Gene Expression in Allergy Model Rats. J Pharmacol Sci. 2008;106(3):325-331.   DOI
25 Ra C, Jouvin MH, Kinet JP. Complete structure of the mouse mast cell receptor for IgE (Fc epsilon RI) and surface expression of chimeric receptors (rat-mouse-human) on transfected cells. J Biol Chem. 1989;264(26):15323-15327.   DOI
26 Theoharidies TC, Kalogeromitros D. The critical role of mast cells in allergy and inflammation. Ann. N.Y. Acad. Sci. 2006;1088:78-99.   DOI
27 Boyce, J.A. Mast cells and eicosanoid mediators: a system of reciprocal paracrine and autocrine regulation. Immunol Rev. 2007;217(1):168-185.   DOI
28 Paolini R, Jouvin MH, Kinet JP. Phosphorylation and dephosphorylation of the high-affinity receptor for immunoglobulin E immediately after receptor engantigenement and disengantigenement. Nature. 1991;353:855-858.   DOI
29 Gilfillan AM, Tkaczyk C. Integrated signalling pathways for mast-cell activation. Nat. Rev. Immunol. 2006;6:218-230.   DOI
30 Jun CD, Pae HO, Kim YC, Jeong SJ, Yoo JC, Lee EJ, Choi BM, Chae SW, Park RK, Chung HT. Inhibition of nitric oxide synthesis by butanolfraction ofthe methanol extract of Ulmus davidiana in murine macrophages. J Ethnopharmacol. 1998;62:129-135.   DOI