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http://dx.doi.org/10.14374/HFS.2021.29.4.155

The Role of Autophagy in Apoptosis Induced by Water Extract of Platycodonis Radix in H460 Human Lung Cancer Cells  

Hong, Su Hyun (Department of Biochemistry, Dong-eui University College of Korean Medicine)
Choi, Yung Hyun (Department of Biochemistry, Dong-eui University College of Korean Medicine)
Publication Information
Herbal Formula Science / v.29, no.4, 2021 , pp. 155-165 More about this Journal
Abstract
Objectives : Recent studies have suggested that Platycodonis Radix has various pharmacological effects such as anti-cancer, antioxidant, anti-asthma, anti-diabetes, anti-obesity, hepatoprotective, and cardiovascular protection effects. The aim of this study was to investigate the role of water extract of Platycodonis Radix (WPR)-induced autophagy in H460 human lung cancer cells. Methods : H460 cells were treated with WPR and cell viability was calculated by an MTT assay. To evaluate changes in apoptosis- and autophagy-related genes, Western blotting was performed. Two kinds of autophagy inhibitors, 3-Methyladenine (3-MA) and bafilomycin A1, were pretreated to confirm the role of WPR-induced autophagy. Results : WPR reduced the viability of H460 cells in a treatment concentration-dependent manner, which was associated with induction of apoptosis. It was also confirmed that WPR induced autophagy based on the formation of specific intracellular vacuoles and changes in the expression of autophagy-related genes. Interestingly, pretreatment with 3-MA and bafilomycin A1 increased WPR-induced cytotoxicity and apoptosis. Conclusions : WPR induced autophagy at low concentrations and early stages of treatment, but promoted apoptosis at high concentrations and late stages. Moreover, WPR-induced autophagy had a cytoprotective role in H460 cells.
Keywords
Apoptosis; Autophagy; H460 lung cancer cells; Platycodonis Radix;
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