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http://dx.doi.org/10.14374/HFS.2021.29.3.127

Anti-inflammatory and Cartilage Protection Effects of Daeganghwal-tang in MIA-induced Osteoarthritis at Rats  

Kim, Ju-ran (Department of Acupuncture & Moxibustion medicine, College of Korean medicine, Daegu Haany University)
Lee, Jung Hee (Department of Acupuncture & Moxibustion medicine, College of Korean medicine, Daegu Haany University)
Lee, Yun Kyu (Department of Acupuncture & Moxibustion medicine, College of Korean medicine, Daegu Haany University)
Lee, Hyun-Jong (Department of Acupuncture & Moxibustion medicine, College of Korean medicine, Daegu Haany University)
Kim, Jae Soo (Department of Acupuncture & Moxibustion medicine, College of Korean medicine, Daegu Haany University)
Publication Information
Herbal Formula Science / v.29, no.3, 2021 , pp. 127-145 More about this Journal
Abstract
Objectives : The purpose of this study was to evaluate the effects of Daeganghwal-tang on knee cartilage in monosodium iodoacetate(MIA)-induced osteoarthritis rats. Methods : Forty SD rats were randomly divided into five groups(n=8/group): normal group was SD rats group injected with normal saline at left knee joint and administrated orally distilled water, control group was MIA-induced osteoarthritis SD rats group administrated orally distilled water, Indomethacin group was MIA-induced osteoarthritis SD rats group administrated orally indomethacin 2 mg/kg, DGHT(L) group was MIA-induced osteoarthritis SD rats group administrated orally 1280 mg/kg of Daeganghwal-tang, and DGHT(H) group was MIA-induced osteoarthritis SD rats group administrated orally 2560 mg/kg of Daeganghwal-tang. After orally administration of drugs for 4 weeks, gross appearance and histological analysis were used to evaluate the degree of knee cartilage damage. In addition, pro-inflammatory cytokines, bone degrade factor and bone defence factors were analyzed to investigate the anti-inflammatory and cartilage protection effects of Daeganghwal-tang. Also, hematological test, biochemical test, and liver and kidney tissue were analyzed to determine the safety of Daeganghwal-tang. Results : Daeganghwal-tang inhibited the damage of the knee cartilage, and significantly prevented the reduction in cartilage thickness. In addition, the pro-inflammatory cytokines and the bone degrade factor significantly decreased, and the bone defence factors significantly increased. In the safety assessment of Daeganghwal-tang, there were no significant differences among the experimental groups and no abnormal findings were observed. Conclusions : Daeganghwal-tang has anti-inflammatory effect, inhibits cartilage damage, and protects cartilage in MIA-induced osteoarthritis rats.
Keywords
Daeganghwal-tang; MIA; Osteoarthritis; Anti-inflammatory effect; Cartilage protection effect;
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