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http://dx.doi.org/10.14374/HFS.2021.29.3.119

Modulation of Fermented Lotus Root on Pacemaker Potentials in Interstitial Cells of Cajal of Murine Small Intestine  

Park, Dong Suk (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
Kim, Jeong Nam (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
Kwon, Hyo Eun (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
Kwon, Min Ji (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
Park, Eun-Jung (Department of Food and Nutrition, Gachon University)
Lee, Hae-Jeung (Department of Food and Nutrition, Gachon University)
Kim, Byung Joo (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
Publication Information
Herbal Formula Science / v.29, no.3, 2021 , pp. 119-125 More about this Journal
Abstract
Obejectives : The purpose of this study is to find out the efficacy of pacemaker potentials of interstitial Cells of Cajal (ICC) by Fermented Lotus Root (FLR) in small intestine. Methods : Enzyme digestions were used to separate the ICC. Using electrophysiological methods, pacemaker potentials were measured and intestinal transit rates (ITR) experiments were conducted to identify in vivo gastrointestinal motility. Results : 1. FLR (0.5-10 mg/ml) caused membrane depolarization by electrophysiological methods. 2. In the case of pretreatment with a Ca2+ free solution and thapsigargin, the pacemaker potential disappeared and in this case, FLR did not have a membrane depolarization reaction. 3. Lowering the concentration of extracellular Na+ concentration stoped the pacemaker potentials and inhibited the reaction caused by FLR. Flufenamic acid also inhibited the reaction by FLR. 4. In mice, ITR was increased by FLR. Conclusions : This study shows that FLR can control ICC by an internal/external Ca2+ and Na+. It also shows that FLR can be a good candidate for gastrointestinal motility medication development.
Keywords
Fermented Lotus Root; Interstitial Cells of Cajal; Gastrointestinal motility; Medication development;
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