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http://dx.doi.org/10.14374/HFS.2020.28.4.327

Hepatocyte protection and antioxidant effect of Citri Unshius Pericarpium against cadmium-induced oxidative stress  

Noh, Gyu Pyo (College of Korean Medicine, Daegu Haany University)
Byun, Sung Hui (College of Korean Medicine, Daegu Haany University)
Jung, Dae Hwa (D&B Bio)
Lee, Jong Rok (Department of Pharmaceutical Engineering, Daegu Haany University)
Park, Sook Jahr (Department of Pharmaceutical Engineering, Daegu Haany University)
Kim, Sang Chan (College of Korean Medicine, Daegu Haany University)
Publication Information
Herbal Formula Science / v.28, no.4, 2020 , pp. 327-337 More about this Journal
Abstract
Objective : Citri Unshius Pericarpium is the dried peel of mature fruit of Citrus unshiu Markovich and has been used to treat indigestion, vomiting, and removal of phlegm. This study investigated the hepatoprotective and antioxidant effect of CEE (Ethanol extract of Citri Unshius Pericarpium) in cadmium (CdCl2)-treated HepG2 cells. Methods : Component analysis of Citri Unshius Pericarpium was analyzed by UPLC with C18 column. Cell viability was determined by MTT assay. The enzyme activity of superoxide dismutase (SOD) and the level of reactive oxygen species (ROS) and reduced glutathione (GSH) were analyzed using commercially available kits. Results : Cadmium caused severe HepG2 cell death. Cadmium also increased ROS production, consistent with depletion of GSH and inhibition of the SOD enzyme. However, CEE treatment reduced cell death and relieved oxidative stress caused by cadmium toxicity. CEE lowered ROS levels and improved depletion of GSH levels. CEE also enhanced the enzymatic activity of SOD. In component analysis, hesperidin was the most abundant of the five marker compounds (Narigenin, Narigin, Narirutin, Hesperidin and Hesperidin), which assumes that hesperidin partially contributed to the antioxidant activity of CEE. Conclusion : These results suggested that CEE could be a potential substance to solve heavy metal-related health problems. In particular, inhibition of oxidative stress by CEE can be a way to treat liver damage caused by cadmium.
Keywords
Citri Unshius Pericarpium; antioxidant; reactive oxygen species; glutathione; superoxide dismutase; hesperidin;
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1 Lee HI, Park AR, Chun SW. Effects of mitochondrial reactive oxygen species on neuronal excitability in rat spinal substantia gelatinosa neurons. Int J Oral Biol. 2012;37(1):17-23.
2 Kwon GR, Moon IS, Lee WC. Neuroprotective effects of Pinelliae rhizoma water-extract by suppression of reactive oxygen species and mitochondrial membrane potential loss in a hypoxic model of cultured rat cortical cells. J Life Sci. 2009;19(5):598-606.   DOI
3 Cichoz-Lach H, Michalak A. Oxidative stress as a crucial factor in liver diseases. World J Gastroenterol. 2014;20(25):8082-91.   DOI
4 Wang L, Yan B, Liu N, Li Y, Wang Q. Effects of cadmium on glutathione synthesis in hepatopancreas of freshwater crab, Sinopotamon yangtsekiense. Chemosphere. 2008;74(1):51-6.   DOI
5 Noctor G, Foyer CH. Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol. 1998;49:249-79.   DOI
6 Romero MJ, Canada AT. The evaluation of Escherichia coli as a model for oxidant stress in mammalian hepatocytes: role of glutathione. Toxicol Appl Pharmacol. 1991;111: 485-95.   DOI
7 Haouem S, Hmad N, Najjar MF, Hani AE, Sakly R. Accumulation of cadmium and its effects on liver and kidney functions in rats given diet containing cadmium-polluted radish bulb. Exp Toxicol Pathol. 2007;59:77-80.   DOI
8 Dkhil MA, Marwa SM, Diab MSM, Lokman MS, El-Sayed H, Aljawdah HMA, Al-Quraishy S, Bauomy AA. Hepato-protective effect of Pleurotus ostreatus extracts in cadmium-intoxicated rats. J King Saud Univ Sci. 2020;32(8):3432-6.   DOI
9 Fridovich I. Superoxide dismutases. Annu Rev Biochem. 1975;44:147-59.   DOI
10 Kim DK, Lee KT, Eun JS, Zee OP, Lim JP, Eum SS, Kim SH, Shin TY. Anti-allergic components from the peels of Citrus unshiu. Arch Pharm Res. 1999;22(6):642-5.   DOI
11 Choi JH, Rhee SJ. Effects of green tea catechin on cadmium accumulation in chronic cadmium poisoned rats. Kor J Nutr. 2001;34(4):384-92.
12 Cho EJ, Li L, Noriko Y, Kim HY. Antioxidative effects of hesperidin and hesperetin under cellular system. CNU J Agric Sci. 2011;38(4):717-22.
13 Imlay JA, Fridovich I. Assay of metabolic superoxide production in Escherichia coli. J Biol Chem. 1991;266(11):6957-65.   DOI
14 Satarug S, Vesey DA, Gobe GC. Health risk assessment of dietary cadmium intake: Do current guidelines indicate how much is safe? Environ Health Perspect. 2017;125:284-8.   DOI
15 Oriental Pharmacology Committee. Oriental Pharmacology. 2nd ed. Seoul:Shinil. 2006:670-5.
16 Leelavinothan P, Kalist S. Hesperetin protects against oxidative stress related hepatic dysfunction by cadmium in rats. Exp Toxicol Pathol. 2012;64(5):513-20.   DOI
17 Nawrot TS, Staessen JA, Roels HA. Cadmium exposure in the population: From health risks to strategies of prevention. Biometals. 2010;23:769-82.   DOI
18 Hung KC. The Pharmacology of Chinese herbs. 2nd ed. Cleveland:CRC Press. 1998:285-8.
19 Hyon JS, Kang SM, Mahinda S, Koh WJ, Yang TS, Oh MC, Oh CK, Jeon YJ, Kim SH. Antioxidative activities of extracts from dried Citrus sunki and C. unshiu peels. J Korean Soc Food Sci Nutr. 2010;39(1):1-7.   DOI
20 Mezynska M, Brzoska MM. Environmental exposure to cadmium-A risk for health of the general population in industrialized countries and preventive strategies. Environ Sci Pollut Res. 2018;25:3211-32.   DOI
21 Brzoska MM, Rogalska J, Roszczenko A, Galazyn-Sidorczuk M, Tomczyk M. The mechanism of the osteoprotective action of a polyphenol-rich Aronia melanocarpa extract during chronic exposure to cadmium is mediated by the oxidative defense system. Planta Med. 2016;82:621-31.   DOI
22 Lin J, Zhang F, Lei Y. Dietary intake and urinary level of cadmium and breast cancer risk: A meta-analysis. Cancer Epidemiol. 2016;42:101-7.   DOI
23 Kang MY, Cho SH, Lim YH, Seo JC, Hong YC. Effects of environmental cadmium exposure on liver function in adults. Occup Environ Med. 2013;70:268-73.   DOI
24 Ramakrishnan R, Elangovan P, Pari L. Protective role of tetrahydrocurcumin: An active polyphenolic curcuminoid on cadmium-induced oxidative damage in rats. Appl Biochem Biotechnol. 2017;183:51-69.   DOI
25 Skipper A, Sims JN, Yedjou CG, Tchounwou PB. Cadmium chloride induces DNA damage and apoptosis of human liver carcinoma cells via oxidative stress. Int J Environ Res Public Health. 2016;13(1):88.   DOI
26 Rani A, Kumar A, Lal A, Pant M. Cellular mechanisms of cadmium-induced toxicity: A review. Int J Environ Health Res. 2013; 24:378-99.   DOI
27 Brzoska MM, Borowska S, Tomczyk M. Antioxidants as a potential preventive and therapeutic strategy for cadmium. Curr Drug Targets. 2016;17:1350-84.   DOI
28 Cha BJ. Changes in the constituents and antioxidant activity in accordance with the processing conditions of Citrus unshiu Markovich. Kor J Pharmacogn. 2015;46(1): 23-30.
29 Borrelli F, Izzo AA. The plant Kingdom as a source of anti ulcer remedies. Phytother Res. 2000;14(8):581-91.   DOI