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http://dx.doi.org/10.14374/HFS.2020.28.1.29

Effects of glucoraphanin in dexamethasone-induced skeletal muscle atrophy in vitro model  

Jeon, Sang Kyu (College of Korean Medicine, Dongguk University)
Kim, Ok Hyeon (College of Korean Medicine, Dongguk University)
Park, Su Mi (College of Korean Medicine, Dongguk University)
Lee, Ju-Hee (College of Korean Medicine, Dongguk University)
Park, Sun-Dong (College of Korean Medicine, Dongguk University)
Publication Information
Herbal Formula Science / v.28, no.1, 2020 , pp. 29-39 More about this Journal
Abstract
Objectives : Glucoraphanin is one of the well-known natural glucosinolates found in cruciferous plants. In the present study, we investigated the effects and molecular mechanism of glucoraphanin in dexamethasone-induced skeletal muscle atrophy in vitro model. Methods : The cytotoxic effects of glucoraphanin on C2C12 myoblasts or myotubes were evaluated by MTT assay. The glucoraphanin was evaluated effects in dexamethasone-induced skeletal muscle atrophy in C2C12 myotubes using a real-time PCR, western blots analysis, and immunofluorescence staining of myosin heavy chain. Result : Glucoraphanin had no cytotoxicity on both C2C12 myoblasts or myotubes. Dexamethasone markedly induced muscle atrophy by up-regulating muscle-specific ubiquitin E3 ligase markers, atrogin-1 and MuRF1, and down-regulating MyoD, a myogenic regulatory factor whereas co-treatment of glucoraphanin and dexamethasone dose-dependently inhibited it. Furthermore, decreased expressions of p-Akt, p-FOXO1, and p-FOXO3a induced by dexamethasone were reversed by co-treatment with glucoraphanin and dexamethasone. In addition, dexamethasone obviously reduced myotube diameters, while co-treatment of glucoraphanin and dexamethasone increased those to a similar level as control. Conclusions : These results show that glucoraphanin suppresses dexamethasone-induced muscle atrophy in C2C12 myotubes through activation of Akt/FOXO signaling pathway.
Keywords
Glucoraphanin; C2C12; dexamethasone; muscle atrophy; Akt; FOXO;
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Times Cited By KSCI : 4  (Citation Analysis)
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