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http://dx.doi.org/10.14374/HFS.2018.26.1.1

Anti-inflammatory and Antioxidant Effects of Cheongnoimyungshin-hwan in RAW 264.7 Macrophages  

Son, Byun Woo (Department of Korean Internal Medicine, College Of Korean Medicine, Dong-Eui University)
Lee, Myeong Hwa (Department of Korean Internal Medicine, College Of Korean Medicine, Dong-Eui University)
Hwang, Won Deok (Department of Korean Internal Medicine, College Of Korean Medicine, Dong-Eui University)
Publication Information
Herbal Formula Science / v.26, no.1, 2018 , pp. 1-12 More about this Journal
Abstract
Objectives : Cheongnoimyungshin-hwan (CNMSH) is a Herbal compound prescription that is composed mainly of herbal medicines such as Ginseng Radix Alba, Angelicae Gigantis Radix, Dioscoreae Rhizoma, Longan Arillus and cornus cervi parvum, and for the purpose of improving memory and preventing dementia. Methods : In this study, it was investigated whether CNMSH could suppress inflammatory response and oxidative stress in the lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. As a result, CNMSH decreased expression of inducible nitric oxide (NO) synthase and cyclooxygenase-2, and also inhibited production of NO, prostaglandin E2. Results : This effect was associated with the suppression of the expression of p65, one of the nuclear factor-kappaB ($NF-{\kappa}B$) subunits, and increased expression of $I{\kappa}B-{\alpha}$, inhibit the $NF-{\kappa}B$ transcription factor. In addition, CNMSH significantly blocked intracellular reactive oxygen species accumulation in response to LPS stimulation. Furthermore, CNMSH increased expression of nuclear factor erythroid 2-related factor (Nrf)-2 activation and heme oxygenase (HO)-1. Conclusions : Therefore, it has been shown anti-inflammatory and antioxidant effects by inhibiting the expression and production of inflammatory mediators in LPS-stimulated macrophages, and is associated with ROS generation and is activated by Nrf2/HO-1 signaling pathway.
Keywords
Cheongnoimyungshin-hwan (CNMSH); Anti-inflammatory and Antioxidant Effects; RAW 264.7 Macrophages;
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