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http://dx.doi.org/10.14374/HFS.2017.25.4.457

Anti-obese Effects and Signaling Mechanisms of Chaenomeles sinensis extracts in 3T3-L1 Preadipocytes and Obese Mice Fed a High-fat Diet  

Kim, Da-Hye (Jeonju AgroBio-Materials Institute)
Kwon, Bora (Jeonju AgroBio-Materials Institute)
Kim, Sang Jun (Jeonju AgroBio-Materials Institute)
Kim, HongJun (Department of Korean Medical Prescription, College of Korean Medicine, Woosuk University)
Jeong, Seung-Il (Jeonju AgroBio-Materials Institute)
Yu, Kang-Yeol (Jeonju AgroBio-Materials Institute)
Kim, Seon-Young (Jeonju AgroBio-Materials Institute)
Publication Information
Herbal Formula Science / v.25, no.4, 2017 , pp. 457-469 More about this Journal
Abstract
Obesity is one of the most serious health problem because it induced numerous metabolic syndrome and increases the incidence of various disease, including diabetes, hypertension, dyslipidemia, atherosclerosis, and cancer. In 3T3-L1 adipocytes, increases in reactive oxygens species (ROS) occur with lipid accumulation. NADPH oxidase, producing superoxide anion, may contribute to the development of obesity-associated insulin resistance and type 2 diabetes. In this study, we elucidated the effect of Chaenomeles sinensis koehne extract (CSE) against the development of obesity and the inhibition mechanisms in 3T3-L1 preadiocytes. CSE decreased triglyceride content and inhibited the expression of adipogenic transcription factors including peroxisome proliferator-activated receptor $(PPAR){\gamma}$, CCAT/enhancer binding protein $(C/EBP){\alpha}$ and sterol regulatory element-binding protein (SREBP-1). In addition, CSE highly increased antioxidant activity in a dose-dependent manner. CSE remarkably reduced intracellular ROS increase and NAD(P)H oxidase activity, NOX1, NOX4, Rac1 protein expression, and phosphorylation of p47phox and p67phox We also studied the effect of CSE on weight gain induced by high-fat diet. The oral treatment of CSE (500 mg/kg, body weight) in diet-induced obese (DIO) mice showed decrease in triglyceride and adipocyte size. Therefore, these results indicate that the effect of CSE on anti-obese effects, adipocyte differentiation and reducing triglyceride contents as well as adipocyte size in obese mice, may be associated with inhibition of NAD(P)H oxidase-induced ROS production and adipose transcription factors. These results showed the potential to inhibit the obesity by CSE treatment through controlling the activation of NAD(P)H oxidase in vitro and in vivo obese model.
Keywords
Chaenomeles sinensis; 3T3-L1; Obesity; NADPH oxidase; reactive oxygen species;
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