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http://dx.doi.org/10.14374/HFS.2017.25.3.349

Study of Signaling Pathway on Apoptotic Cell Death Induced by Extract of Ailanthus altissima in Human Jurkat Lymphocytes  

Lee, Ki Ouk (Graduate School of Wonkwang University)
Kim, Ae Wha (Graduate School of Wonkwang University)
Lim, Kyu Sang (Professional Graduate School of Korean Medicine, Wonkwang University)
Yun, Young Gab (Graduate School of Wonkwang University)
Publication Information
Herbal Formula Science / v.25, no.3, 2017 , pp. 349-362 More about this Journal
Abstract
Objectives : We investigated whether the components of Ailanthus altissima induced apoptotic cell death in Jurkat acute lymphoblastic leukemia (ALL) cells. Methods : Regulation of cell proliferation is a complex process involving the regulated expression and/or modification of discrete gene products, which control transition between different stages of the cell cycle. Results : Upon treatments with Ailanthus altissima, the concentration-dependent inhibitions of cell viability were observed as compared to untreated control group. The capability of Ailanthus altissima to induce apoptosis was associated with proteolytic cleavage of specific target proteins such as poly(ADP-ribose)polymerase (PARP) and beta-catenin proteins suggesting the possible involvement of caspases. Ailanthus altissima also caused apoptosis as measured by cell morphology and DNA fragmentation. Conclusions : These results indicate that the increase of apoptotic cell death by Ailanthus altissima may be due to the inhibition of cell cycle in human Jurkat lymphocytes. Conclusively, these current and further findings will provide novel approaches to understanding and treating major diseases.
Keywords
Ailanthus altissima; Apoptotic Cell Death; Human Jurkat Lymphocytes;
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