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http://dx.doi.org/10.14374/HFS.2015.23.2.199

Induction of Cell Cycle Arrest at G2/M phase by Ethanol Extract of Scutellaria baicalensis in Human Renal Cell Carcinoma Caki-1 Cells  

Park, Dong-Il (Department of Internal Medicine, Dongeui University College of Korean Medicine)
Jeong, Jin-Woo (Anti-Aging Research Center, Dongeui University)
Park, Cheol (Department of Molecular Biology, College of Natural sciences & Human Ecology, Dongeui University)
Hong, Su-Hyun (Department of Biochemistry, Dongeui University College of Korean Medicine)
Shin, Soon-Shik (Department of Formula Sciences, Dongeui University College of Korean Medicine)
Choi, Sung-Hyun (Department of Safety & System Management, Korea Lift College)
Choi, Yung-Hyun (Anti-Aging Research Center, Dongeui University)
Publication Information
Herbal Formula Science / v.23, no.2, 2015 , pp. 199-208 More about this Journal
Abstract
Objectives : In the present study, we investigated the effects of ethanol extract of Scutellaria baicalensis (EESB) on the progression of cell cycle in human renal cell carcinoma Caki-1 cells. Methods : The effects of EESB on cell growth and apoptosis induction were evaluated by trypan blue dye exclusion assay and flow cytometry, respectively. The mRNA and protein levels were determined by Western blot analysis and reverse transcription-polymerase chain reaction, respectively. Results : It was found that EESB treatment on Caki-1 cells resulted in a dose-dependent inhibition of cell growth and induced apoptotic cell death as detected by Annexin V-FITC staining. The flow cytometric analysis indicated that EESB resulted in G2/M arrest in cell cycle progression which was associated with the down-regulation of cyclin A expression. Our results also revealed that treatment with EESB increased the mRNA and proteins expression of tumor suppressor p53 and cyclin-dependent kinase (Cdk) inhibitor p21(WAF1/CIP1), without any noticeable changes in cyclin B1, Cdk2 and Cdc2. In addition, the incubation of cells with EESB resulted in a significant increase in the binding of p21 and Cdk2 and Cdc2. These findings suggest that EESB-induced G2/M arrest and apoptosis in Caki-1 cells is mediated through the p53-mediated upregulation of Cdk inhibitor p21. Conclusions : Taken together, these findings suggest that EESB may be a potential chemotherapeutic agent and further studies will be needed to identify the biological active compounds that confer the anti-cancer activity of S. baicalensis.
Keywords
Scutellaria baicalensis L.; renal cell carcinoma Caki-1 cells; G2/M arrest; apoptosis; p21;
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