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http://dx.doi.org/10.14374/HFS.2013.21.1.071

Simultaneous Analysis of Six Compounds in Yijin-tang by HPLC-PDA  

Kim, Seong-Sil (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine)
Kim, Jung-Hoon (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine)
Seo, Chang-Seob (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine)
Publication Information
Herbal Formula Science / v.21, no.1, 2013 , pp. 71-79 More about this Journal
Abstract
Objectives : Yijin-tang has been used in the treatment of gastrointestinal diseases such as irritable bowel syndrome, gastritis, and gastric ulcer. In this study, a high-performance liquid chromatography (HPLC) method was established for simultaneous analysis of six compounds, liquiritin, glycyrrhizin, hesperidin, 6-gingerol, homogentisic acid, and 3,4-dihydroxybenzaldehyde in Yijin-tang, a traditional Korean herbal medicinal preparation. Methods : A Gemini C18 column was used for the separation of six constituents at $40^{\circ}C$. The mobile phase using gradient elution consist of two solvent systems, 1.0% acetic acid in water (A) and 1.0% acetic acid in acetonitrile (B). The flow-rate was 1.0 mL/min and injection volume was $10{\mu}g$. The detector was a photodiode array (PDA) detector set at 254 nm and 280 nm. Results : The calibration curves of six compounds showed good linearity in various concentration ranges ($R^2{\geq}0.9997$). The limits of detection (LOD) and limits of quantification (LOQ) were 0.028-$0.192{\mu}g/mL$ and 0.093-$0.540{\mu}g/mL$, respectively. The RSD (%) of the intra and inter day validations were 0.03-0.84% and 0.05 -1.00%, respectively. Recovery was 96.14-01.90% and RSD (%) was less than 1.5%. Conclusions : The established simultaneous analysis methods will help management to improve the quality of Yijin-tang.
Keywords
Yijin-tang; HPLC-PDA; Validation; Simultaneous determination;
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Times Cited By KSCI : 1  (Citation Analysis)
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