Browse > Article
http://dx.doi.org/10.14374/HFS.2012.20.2.093

Acute Toxicity Study on Gumiganghwal-tang and Fermented Gumiganghwal-tang Extracts  

Park, Hwayong (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
Hwang, Youn-Hwan (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
Jang, Doorye (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
Ha, Jeong-Ho (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
Jung, Kiyoun (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
Ma, Jin Yeul (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
Publication Information
Herbal Formula Science / v.20, no.2, 2012 , pp. 93-102 More about this Journal
Abstract
Objectives : Traditional medicine Gumiganghwal-tang (GT) has been used in Asia to treat inflammatory diseases including common cold, pain, fever, and algor. In this study we investigated the acute toxicity and safety of GT and fermented GT (FGT). Methods : Acute toxicity and safety were evaluated in male and female ICR mice orally administered 0 (control) and 2,000 mg/kg of GT and FGT. After the administration of GT and FGT, we observed mortality, body weight, clinical symptoms. After necropsy, organ weights were measured and blood analysis was performed. Results : There was no mortality and clinical symptoms according to the administration of GT and FGT. Comparing with control group, there were no significant alterations on the organ weight, complete blood cell count and biochemical parameters. Conclusions : Median lethal dose of GT and FGT considered to be over 2,000 mg/kg in both male and female mice, and recognized as safe with no toxicity.
Keywords
Gumiganghwal-tang; Fermentation; Acute toxicity;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 허준. 동의보감. 서울:민중서원. 1993:669.
2 한의과대학 방제학교수 공편저. 방제학. 서울: 영림사. 1999:74-5.
3 조재국. 한방이용 및 한약소비 실태조사. 서울:한국보건사회연구원. 2011:260.
4 허승철, 정희재, 정승기, 이형구. 구미강활탕의 효능에 관한 실험적 연구. 경희의학. 1998;14(2) :174-83.
5 문영희, 고재종, 박조영. 구미강활탕의 소염 진통작용. 생약학회지. 1999;30(1):18-24.
6 Kim SJ, Jeong HJ, Moon PD, Lee KM, Lee HB, Jung HJ, Jung SK, Rhee HK, Yang DC, Hong SH, Kim HM. Anti-inflammatory activity of Gumiganghwaltang through the inhibition of nuclear factor-${\kappa}B$ activation in peritoneal macrophages. Biol Pharm Bull. 2005;28(2):233-7.   DOI
7 Jiang D. Management of migraine using traditional Chinese medicine. J Chin Med. 2004;76:40-4.
8 Baibado JT, Chui KWJ, Cheung HY. Nonprescription antipyretics. Hong Kong Pharmaceut J. 2011;18(3):110-6.
9 이진아, 하혜경, 정다영, 이호영, 이준경, 황대선, 신현규. 한약 처방 25종에 대한 항염증 효능 비교 연구. 대한한방부인과학회지. 2010;23(3):101-11.
10 신인식, 김정훈, 하혜경, 서창섭, 이미영, 이호영, 이준경, 이남헌, 이진아, 이설림, 허정임, 신현규. Spargue-Dawley 랫드를 이용한 구미강활탕의 급성독성 연구. 대한한의학방제학회지. 2010;18(1):79-85.
11 Lee MY, Shin IS, Seo CS, Kim JH, Ha H, Huh JI, Shin HK. A 4-week repeated dose oral toxicity and cytotoxicity study of Gumiganghwaltang in Crl:CD(SD) rats. Toxicol Int. 2011;18(2) :146-54.   DOI
12 Lee MY, Seo CS, Shin IS, Ha H, Kim JH, Cho JW, Huh JI, Shin HK. Toxicological evaluation of Gumiganghwaltang aqueous extract in Crl:CD(SD) rats: 13 weeks oral gavage studies. Regul Toxicol Pharmacol. 2012;62:553-60.   DOI
13 Shin IS, Seo CS, Lee MY, H HK, Huh JI, Shin KH. In vitro and in vivo evaluation of the genotoxicity of Gumiganghwal-tang, a traditional herbal prescription. J Ethnopharmacol. 2012;141 :350-6.   DOI
14 이정규, 우원식. 강활의 쿠마린 성분. 생약학회지. 1982;13(1):10-3.
15 권용수, 우은란, 김창민. 강활의 생물활성분획에 대한 성분연구. 생약학회지. 1991;22(3):156-61.
16 권용수, 인고길, 김창민. 강활의 성분. 생약학회지. 2000;31(3):284-7.
17 김소준, 진영원, 윤기동, 류민열, 양민혜, 이제현, 김진웅. 방풍의 화학 성분. 생약학회지. 2008; 39(4):357-64.
18 Santos MM, Piccirillo C, Castro PM, Kalogerakis N, Pintado ME. Bioconversion of oleuropein to hydroxytyrosol by lactic acid bacteria. World J Microbiol Biotechnol. 2012;28(6):2435-40.   DOI
19 Chui CH, Hau DK, Lau FY, Cheng GY, Wong RS, Gambari R, Kok SH, Lai KB, Teo IT, Leung TW, Higa T, Ke B, Tang JC, Fong DW, Chan AS. Apoptotic potential of the concentrated effective microorganism fermentation extract on human cancer cells. Int J Mol Med. 2006;17(2):279-84.
20 Jung BG, Ko JH, Cho SJ, Koh HB, Yoon SR, Han DU, Lee BJ. Immune-enhancing effect of fermented Maesil (Prunus mume Siebold & Zucc.) with probiotics against Bordetella bronchiseptica in mice. J Vet Med Sci. 2010;72(9):1195-202.   DOI
21 Jung YM, Lee SH, Lee DS, You MJ, Chung IK, Cheon WH, Kwon YS, Lee YJ, Ku SK. Fermented garlic protects diabetic, obese mice when fed a high-fat diet by antioxidant effects. Nutr Res. 2011;31(5):387-96.   DOI
22 차재영, 전방실, 이치형, 유기수, 문재철, 조영수. Streptozotocin 유발 당뇨쥐에서 발효 차가 버섯의 항당뇨 및 항산화 효과. 생명과학회지. 2005;15(5):809-18.
23 함성호, 임병락, 유가화, 가선오, 박병현. 발효에 의한 오가피의 항당뇨 활성 촉진. 동의생리병리학회지. 2008;22(2):340-5.
24 김형석, 한효상, 이영종. 고삼 발효 추출물의 면역활성에 관한 연구. 대한본초학회지. 2011; 26(2):17-23.
25 Tang JL, Liu BY, Ma KW. Traditional Chinese Medicine. Lancet. 2008;372(9654):1938-40.   DOI