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http://dx.doi.org/10.14374/HFS.2012.20.1.041

Anti-inflammatory effects of HC001 on atopic dermatitis-like skin lesions in mice  

Choi, You-Youn (Dept. of Physiology, College of Oriental Medicine, Kyung-Hee University)
Kim, Mi-Hye (Dept. of Physiology, College of Oriental Medicine, Kyung-Hee University)
Kum, Chang-Jun (Dept. of Anatomy, College of Oriental Medicine, Kyung-Hee University)
Choi, Young-Jin (Dept. of Anatomy, College of Oriental Medicine, Kyung-Hee University)
Hwang, Man-Ki (Dept. of Anatomy, College of Oriental Medicine, Kyung-Hee University)
Sohn, Young-Joo (Dept. of Anatomy, College of Oriental Medicine, Kyung-Hee University)
Jung, Hyuk-Sang (Dept. of Anatomy, College of Oriental Medicine, Kyung-Hee University)
Yang, Woong-Mo (Dept. of Physiology, College of Oriental Medicine, Kyung-Hee University)
Publication Information
Herbal Formula Science / v.20, no.1, 2012 , pp. 41-49 More about this Journal
Abstract
Objectives : The purpose of this study is to examine the effects of external application of HC001 on atopic dermatitis. HC001, newly composed herbal medicine combinations contains 9 medicinal herbs which are known to have anti-inflammatory and anti-allergic effects. We investigated the anti-inflammatory effects of HC001 on atopic dermatitis-like skin lesions in mice. Methods : Seven-week-old BALB/c mice were sensitized with DNCB to develop atopic dermatitis. Animals were divide into three group: Normal, DNCB (Negative control group), HC001 (Experimental group, treated with DNCB and HC001). Skin sections were stained with H&E to measure the thicknesses of the epidermis and dermis, respectively. The expression of NF-${\kappa}B$ protein was measured by western blotting analysis in skin lesion. Results : Topical HC001 treatment significantly restored the skin thickening and hyperplasia of the epidermis and dermis compared with DNCB-sensitized group in histopathological analyse. In addition, HC001 inhibited the expression of NF-${\kappa}B$ protein increased in DNCB-induced atopic dermatitis-like skin lesions in mice. Conclusions : These results suggest that HC001 may be useful as an external application agent for atopic dermatitis based on reductions of various inflammatory responses.
Keywords
Atopic dermatitis; Anti-inflammation; HC001; NF-${\kappa}B$;
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Times Cited By KSCI : 3  (Citation Analysis)
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