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Antiinflammatory Effect of Bioactive Compounds from Geranium nepalense (Notes)  

Kim, Bo-Mi (Division of Nanobiochemistry, College of Natural Sciences, Wonkwang University)
Kim, Eui-Sung (Eco-Friendly Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology)
Lee, Young-Hang (Division of Nanobiochemistry, College of Natural Sciences, Wonkwang University)
Yu, Byung-Soo (Division of Nanobiochemistry, College of Natural Sciences, Wonkwang University)
Chai, Kyu-Yun (Division of Nanobiochemistry, College of Natural Sciences, Wonkwang University)
Publication Information
Herbal Formula Science / v.18, no.2, 2010 , pp. 241-249 More about this Journal
Abstract
The Geranium nepalense has been used traditionally for treatment of various diseases. However, the molecular studies on the effect of Geranium nepalense have not been carried out. In the present study, Quercetin, quercitrin, and afzelin were isolated from the methanol extract of Geranium nepalense were tested for their anti-inflammatory effect. The anti-inflammatory effect of the compounds was studied in lipopolysaccharide(lps)-treated mouse macrophage cells, RAW 264.7. RAW 264.7 cells were pre-incubated with isolated compounds(0, 5, 10, 20, 40, $50\;{\mu}g/ml$) for 4h and treated with $1\;{\mu}g/ml$ lps for 18h, and then the anti-inflammatory effects of compounds were determined. The results are as follows: Quercetin at various concentration inhibited the viability of Raw 264.7 from 7% to 45%, quercitrin from 25% to 80%, and afzelin from 13% to 52%. Isolated compounds showed a significant decrease in iNOS (inducible nitric oxide synthase) and COX-2 (cyclooxygenase-2). These results suggest that these compounds can be used as stable anti-inflammatory materials.
Keywords
Quercetin; quercitrin; afzelin; Geranium nepalense; nitric oxide; prostaglandin E2; inducible nitric oxide synthase; cyclooxygenase-2;
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Times Cited By KSCI : 3  (Citation Analysis)
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