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http://dx.doi.org/10.5407/JKSV.2017.15.3.041

Visualization on the Functional Changes of Endothelial Cells Due to Apoptotic Macrophage in Atherosclerosis Microenvironment  

Kim, Wanho (epartment of Mechanical Engineering, KAIST)
Son, Jegoo (epartment of Mechanical Engineering, KAIST)
Jeon, Jessie Sungyun (epartment of Mechanical Engineering, KAIST)
Publication Information
Journal of the Korean Society of Visualization / v.15, no.3, 2017 , pp. 41-46 More about this Journal
Abstract
The apoptosis of macrophages occurs throughout all stages of atherosclerosis. It is known to constitute atheromatous plaque, increase plaque instability, and thus contribute to the development of atherosclerosis. However, there still remains much to be elucidated on how the apoptotic macrophages affect the endothelial cells and also how they contribute to the development of atherosclerosis. Here we present a microfluidic system, which enables co-culture of apoptotic macrophages and endothelial cells in fibrin gel that mimics in vivo extracellular matrix. With the system, we can investigate the effect of macrophage apoptosis on vascular endothelial cells by quantitatively analyzing the level of reactive oxygen species of HUVECs, integrity of VE-cadherin and cell proliferation. We expect that this system could be utilized further for understanding different mechanisms of apoptotic macrophage on the development of atherosclerosis.
Keywords
Atherosclerosis; Microfluidics; Endothelial Cell; Macrophage;
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