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http://dx.doi.org/10.5407/JKSV.2010.8.4.048

Visualization of Water-uptake Process in Excised Roots of Arabidopsis using Synchrotron X-ray Imaging Technique  

Hwang, Bae-Geun (POSTECH 기계공학과 대학원)
Kim, Hae-Koo (POSTECH 생물공학연구소)
Lee, Sang-Joon (POSTECH 기계공학과)
Publication Information
Journal of the Korean Society of Visualization / v.8, no.4, 2010 , pp. 48-53 More about this Journal
Abstract
Water-uptake through roots, is an essential process of the water flow in plants. Its visualization is very useful for understanding sap flow dynamics at whole plant level. In this study, the tips of Arabidopsis' root hairs were excised and exposed to repeated dehydration and rehydration processes. The water-refilling through individual xylem vessels was visualized using the synchrotron X-ray micro-imaging technique. The high temporal resolution ($2\;{\mu}m$) and beam intensity of the X-ray source allowed to acquisition of consecutive X-ray images of the water-refilling process up to 10 frames/sec. Various flow patterns were observed and the ascending speed of the water-air interfaces was analyzed. The relation between the water-rising height and ascending speed was also analyzed. The present results would provide better alternative for investigating sap flows in roots.
Keywords
Synchrotron X-ray; Arabidopsis; Root hai; Xylem vessel;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Caemmerer, S., Baker, N., 2007, “The Biology of Transpiration. From Guard Cells to Globe”, Plant Physiology, Vol. 143, pp. 3   DOI
2 Steudle, E., Peterson, C., 1998, “Review Article: How does water get through roots?”, Journal of Experimental Botany, Vol. 49(322), pp. 775-788   DOI   ScienceOn
3 Garrigues, E., Doussan, C., Pierret, A., 2006, “Water uptake by plant roots: I-formation and propagation of a water extraction”, Plant and Soil, Vol. 283, pp. 83-98   DOI
4 Windt, C., Gerkema, E., As, H.V., 2009, “Most water in the tomato truss is imported through the xylem, not the phloem: A nuclear magnetic resonance flow imaging study”, Plant Physiology, Vol. 151, pp. 830-842   DOI   ScienceOn
5 Lee, S.J., Kim, Y.M., 2008, “In vivo visualization of the water-refilling process in xylem vessels using X-ray micro-imaging”, Annals of Botany, Vol. 101, pp. 595-602   DOI   ScienceOn
6 Lee, S.J., Kim, Y.M., 2003, “In vivo visualization of flow in xylem vessels of a bamboo leaf using synchrotron X-ray micro-imaging technique”, Journal of KSME B, Vol. 27(11), pp. 1612-1617   과학기술학회마을   DOI   ScienceOn
7 Lee, S.J., Kim, G.B., Kim, S., Kim, Y.M., 2005, “Development of X-ray PIV technique and its application”, Journal of the Korean Society of Visualization, Vol. 3(1), pp. 20-25
8 Kim, H.K., Lee, S.J., 2010, “Synchrotron X-ray imaging for nondestructive monitoring of sap flow dynamics through xylem vessel elements in rice leaves”, New Phytologist, Vol. 188, Issue 4, pp. 1085-1098   DOI   ScienceOn
9 Ellerby, D.J., Ennos, A.R, 1998, “Resistances to fluid flow of model xylem vessels with simple and scalariform perforation plates”, Journal of Experimental Botany, Vol. 49(323), pp. 929-985