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Gonadal Maturation and Spawning of River Puffer Takifugu obscurus Indoor Cultured in Low Salinity  

Kang, Hee-Woong (West Sea Fisheries Research Institute, National Fisheries Research & Development Institute)
Chung, Ee-Yung (Korea Marine Environment & Ecosystem Rearch Institute)
Kang, Duk-Young (West Sea Fisheries Research Institute, National Fisheries Research & Development Institute)
Park, Young-Je (West Sea Fisheries Research Institute, National Fisheries Research & Development Institute)
Jo, Ki-Che (West Sea Fisheries Research Institute, National Fisheries Research & Development Institute)
Kim, Gyu-Hee (West Sea Fisheries Research Institute, National Fisheries Research & Development Institute)
Publication Information
Journal of Aquaculture / v.21, no.4, 2008 , pp. 331-338 More about this Journal
Abstract
Monthly changes in the gonadosomatic index (GSI) and hepatosomatic index (HSI) of wild river puffer Takifugu obscurus, and water quality environment in spawning area during breeding season were investigated from March 1995 to February 1996. Monthly changes in GSI and HSI of T. obscurus, that was cultured in low salinity, were calculated. The external morphology of the gonads, germ cell differentiation during gametogenesis and the reproductive cycle with the gonad developmental phases were investigated by histological analysis. The optimum water quality environment in Ganggyung, Choongcheongnam-do, where is spawning ground of wild T. obscurus, was $15-20^{\circ}C$ (water temperature) and 0 psu (salinity). Monthly changes in the GSI in females and males reached a maximum in May, and then rapidly decreased. Therefore, it is assumed that in the natural condition the spawning period of wild T. obscurus is May to June. In females and males, it showed a negative correlationship between the GSI and HSI. The external morphology of the gonads in female and male T. obscurus, that was cultured in low salinity, is composed of a pair of saccular structure. Based on monthly changes in the GSI, it is assumed that in female T. obscurus, that was cultured in low salinity, spawn from March through May. Therefore, it showed a negative correlationship between changes in the GSI and HSI. On the whole, in females and males, it showed a similar pattern between wild and cultured T. obscurus. The reproductive cycle with the gonad developmental phases can be classified into successive five stages in females: the early growing stage, late growing stage, mature stage, ripe and spent stage, and recovery and resting stage. In males, that can be divided into successive four stages: the growing stage, mature stage, ripe and spent stage, and recovery and resting stage. In case of wild T. obscurus, the spawning period has once a year, however, those cultured in the high water temperature ($20-27^{\circ}C$) - low salinity (under 3.3 psu) condition have reproductive characteristics having possibilities of discharge of eggs and sperms year-round as a multiple spawner.
Keywords
Takifugu obscurus; GSI; HSI; Maturation; Spawning;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Aida, 1991. Environmental regulation of reproductive rhythms in teleostes. Bull. Inst. Zool.. Acad. Sin. Monograph, 16, 173−187
2 Akira, K., D. Hiroyuki, N. Tsutomu, S. Harumi and H. Shigehisa, 2005. Takifugu obscurus is a euryhaline fugu species very close to Takifugu rubripes and suitable for studying osmoregulation. BMC Physiology, 5, 1−11   DOI   ScienceOn
3 Cha, H. K., Y. M. Choi, J. H. Park, J. Y. Kim and M. H. Sohn, 2002. Maturation and spawning of the chub mackerel, Scomber japonicus Houttuyn in korean waters. J. Kor. Soc. Fish. Res., 5, 24−33
4 Jiang, R. L., C. W. Zhang, Y. K. Ding, C. B. Chen and M. F. Li, 2000. Artificial propagation of nontoxic Takifugu obscurus cultured in pond. J. Fish. China, 24, 539−545
5 Yang, S. G., Y. D. Lee and C. K. Pyen, 1994. A study on the gonadal maturation and egg-stripping by hormone treatments of tiger puffer, Takifugu rubripes. J. Aquacult., 7, 189−205
6 강희웅, 2002. 쥐노래미 Hexagrmmos otakii의 번식생태 및 자치어의 성장. 군산대학교대학원 박사학위논문, 134 pp
7 장선일, 한형균, 강희웅, 서형철, 이권혁, 김문환, 라시환, 1995. 황복 種苗生産 技術開發. 서수연사업보고서, pp. 292−316
8 Asahina, K. and I. Hanyu, 1983. Role of temperature and photoperiod in annual reproductive cycle of the rose bittering, Rhodeus ocellatus ocellatus. Bull. Jap. Soc. Sci. Fish., 49, 61−67
9 Kim, W. S., J. K. Jeon, S. H. Lee and H. T. Huh, 1996. Effects of pentachlorophenol(PCP) on the oxygen consumption rate of the river puffer fish Takifugu obscurus. Mar. Ecol. Prog. Ser., 143, 9−14
10 Park, I. S., H. S. Kim, E. S. Kim, J. H. Kim and C. W. Park, 1997. Cytogenetic analysis of river puffer, (Teleostomi : Tetraodontiformes). J. Kor. Fish. Soc., 30, 408−412
11 Yang, Z. and Y.F. Chen, 2005. Effect of temperature on incubation period and hatching success of obscure puffer Takifugu obscurus (Abe) eggs. Aquaculture, 246, 173−179
12 Kang, H. W., J. H. Lee and J. Y. Lee, 2006. Manual of river puffer aquaculture. NFRDI, 194 pp
13 Kang, H. W, D. Y. Kang, K. C. Cho, J. H. Lee, K. J. Park and J. H. Kim, 2004a. Effect of food and salinity on larval growth and survival of the river puffer, Takifugu obscurus. J. Aquacult., 17, 221−227
14 Kim, W. S., J. M. Kim, S. K. Yi and H. T. Huh, 1997. Endogenous circadian rhythm in the river puffer fish Takifugu obscurus. Mar. Ecol. Prog. Ser., 153, 293−298
15 Yang, Z. and Y.F. Chen, 2003. Induced ovulation using LHRHa in anadromous obscure puffer Takifugu obscurus cultured entirely in freshwater. Fish Physiol. Biochem., 29, 323−326
16 Jang, S. L, H. W. Knag and H. K. Han. 1996. Embryonic, larval and juvenile stages in yellow puffer, Takifugu obscurus. J. Aquacult., 9, 11−18
17 Lim, H. K., S. G. Byun, J. H. Lee, S. U. Pa rk, Y. C. Kim, H. K. Han, B. H. Min and B. Y. Lee, 2007. Sexual maturity and reproductive cycle of starry flounder Platichthys stellatus cultured indoor tank. J. Aquacult., 20, 212−218   과학기술학회마을
18 Shimizu, A. and I. Hanyu, 1993. Factors involved in the development of and decline on photoperiodism as it relates to the gonadal activity of a spring-spawning bitterling, Acheilognathus tabira. J. Exp. Zool., 265, 134−143
19 최기호, 2007. 한국산 짱뚱어, Boleophthalmus pectinirostris (Linnaeus)의 번식생태 및 유전학적 분석. 군산대학교대학원 박사학위논문, 128 pp
20 Kang, H. W., K. B. Shim, D. Y. Kang, K. C. Jo, K. C. Song, J. H. Lee, H. I. Song, S. G. Son and Y. J. Cho, 2007. Sitological quality evaluation of cultured and wild river puffer, Takifugu obscurus (Abe). J. Aquacult., 20, 147−153   과학기술학회마을
21 陽淸發, 2002. 河豚養殖利用. 金盾出版社, 200 pp
22 Chang, Y. J., H. K. Han, Y. J. Chang, H. S. Kim and H. T. Hur, 1999b. Physico-chemical properties and cold storage of river puffer (Takifugu obscurus) milt. J. Kor. Fish. Soc., 32, 243−246
23 Chan, Y. J., H. K. Lim, Y. J. Chang and H. S. Kim, 1999a. Sperm cryopreservation and fertility of post-thaw sperm in river puffer, Takifugu obscurus. J. Aquacult., 12, 1−5
24 Kang, H. W, E. Y. Chung and J. H. Kim, 2004b. Sexual maturation and spawning characteristics in greenling, Hexagrmmos otakii of the west coast in korea. J. Aquacult., 17, 30−38