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Expression of Prolactin Receptor mRNA and Blood Physiological Responses to Salinity Changes in the Black Porgy Acanthopagrus schlegeli  

An, Kwang-Wook (Division of Marine Environment & Bioscience, Korea Maritime University)
Min, Byung-Hwa (Aquaculture Research Team, NFRDI)
Park, In-Seok (Division of Marine Environment & Bioscience, Korea Maritime University)
Heo, Youn-Seong (Aquaculture Research Team, NFRDI)
Choi, Yong-Ki (Division of Marine Environment & Bioscience, Korea Maritime University)
Jo, Pil-Gue (Division of Marine Environment & Bioscience, Korea Maritime University)
Chang, Young-Jin (Department of Aquaculture, Pukyong National University)
Choi, Cheol-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
Publication Information
Journal of Aquaculture / v.21, no.2, 2008 , pp. 63-69 More about this Journal
Abstract
We isolated complementary DNA(cDNA) encoding prolactin receptor(PRLR) from gill of black porgy Acanthopagrus schlegeli. Its PRLR cDNA consists of 1,611 base pairs and encodes the protein of 536 amino acids. To investigate the osmoregulatory abilities of black porgy in different salinities(35, 10 and 0 psu), we examined the expression of PRLR mRNA in osmoregulatory organs(gill, kidney and intestine) using reverse transcription(RT)-PCR. In gill and intestine, PRLR mRNA levels were high in 10 psu, and then decreased in 0 psu, but there is no changes in kidney. Also, plasma osmolality, $Na^+\;and\;Cl^-$ levels decreased during the experimental period. These results suggest that PRLR plays an important role in hormonal regulation in osmoregulatory organs during freshwater acclimation, thereby improving the hyper-osmoregulatory ability of black porgy in hypoosmotic environments.
Keywords
Black porgy; Acanthopagrus schlegeli; freshwater acclimation; osmolality; prolactin receptor;
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