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Biological Characteristics of Scuticociliate, Philasterides dicentrarchi Isolated from Cultured Olive Flounder, Paralichthys olivaceus  

Jin, Chang-Nam (Jindo Maritime Affairs and Fisheries Office)
Kang, Hyun-Sil (Faculty of Marine Science, Cheju National University)
Lee, Chang-Hoon (Jeju Fisheries Resource Institute NFRKE)
Lee, Young-Don (Faculty of Marine Science, Cheju National University)
Lee, Je-Hee (Faculty of Marine Science, Cheju National University)
Heo, Moon-Soo (Faculty of Marine Science, Cheju National University)
Publication Information
Journal of Aquaculture / v.20, no.2, 2007 , pp. 106-113 More about this Journal
Abstract
Biological characteristics of Philasteridies dicentrarchi (Ciliophora:Scuticociliate) isolated from the cultured olive flounder Paralichthys olivaceus was determined out by culture, growth conditions, and in vitro relief effect of chemical compounds. The scuticociliate has an active propagation ability by utilizing organic matters obtained from cell strain, bacteria, assorted feed, brain tissue and rotifer tissue. The ciliate achieved population growth activity under the conditions of $5{\sim}45\;ppt$ in salinity and pH 6-9. The ciliate had survived and propagated under the water temperature ranging $10{\sim}30^{\circ}C$, but active growth was observed in the temperature ranges of $10{\sim}25^{\circ}C$. Therapeutic trials were performed with formalin and hydrogen peroxide. The extermination time of the parasites with formalin was in 30 minutes both at 300 and 400 ppm, 60 minutes at 200 ppm, 90 minutes at 100 ppm, and 120 minutes at 50 ppm, respectively. In hydrogen peroxide treatments extermination time was 60 minutes in 300 ppm, 90 minutes in 200 ppm, and 150 minutes at 150 ppm and 100 ppm concentrations.
Keywords
Scuticociliate; Philasterides dicentrarchi; Olive flounder; Growth conditions; Relief effect;
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1 Crosbie, P. B. B. and B. L. Munday, 1999. Environmental factors and chemical agents affecting the growth of the pathogenic marine ciliate Uronema nigricans. Dis. Aquat. Org., 36, 213-219   DOI
2 Iglesias, R., A. Paramá, M. F. Alvarez, J. Leiro, C. Aja and M. L. Sanmartín, 2003. In vitro growth requirements for the fish pathogen Philasterides dicentrarchi (Ciliophora, Scuticociliatida). Vet. Parasitol., 111, 19-30   DOI   ScienceOn
3 Jee, B. Y. and M. R. Jo, 2002. Trials for the control of scuticociliatosis in the cultured olive flounder (Paralichthys olivaceus) by bath treatment. J. Fish Pathol., 15, 93-97
4 Jee, B. Y., Y. C. Kim and M. S. Park, 2001. Morphology and biology of parasite responsible for scuticociliatosis of cultured olive flounder Paralichthys olivaceus. Dis. Aquat. Org., 47, 49-55   DOI
5 Jin, C. N., C. H. Lee, S. P. Oh, Y. U. Jung, C. B. Song, J. Lee and M. S. Heo, 2003. Scuticociliatosis in flounder farms of Jeju island. J. Fish Pathol., 16, 135-138
6 Novotny, M. J., R. J. Cawthorn and B. Despres, 1996. In vitro effects of chemotherapeutants on the lobster parasite Anophryoides haemophila. Dis. Apuat. Org., 24, 233-237   DOI
7 Dragesco, A., J. Dragesco, F. Coste, C. Gasc, B. Romestand, J. C. Raymond. and G. Bouix, 1995. Philasterides dicentrarchi, n. sp. (Ciliophora, Scuticociliatida), a histophagous opportunistic parasite of Dicentrarchus labrax (Linnaeus, 1758), a reared marine fish. Europ. J. Protistol., 31, 327-340   DOI   ScienceOn
8 Jung, S. J., S. I. Kitamura, J. Y. Song, I. Y. Joung and M. J. Oh, 2005. Complete small subunit rRNA gene sequence of the scuticociliate Miamiensis avidus pathogenic to olive flounder Paralichthys olivaceus. Dis. Aquat. Org., 64, 159-162   DOI
9 Jung, S. J., S. I. Kitamura, J. Y. Song, and M. J. Oh, 2007. Miamiensis avidus (Ciliophora: Scuticociliatida) causes systemic infection of olive flounder Paralichthys olivaceus and is a senior synonym of Philasterides dicentrarchi. Dis. Aquat. Org., 73, 227-234   DOI
10 Provasoli, L., J. J. A. Mclaughlin and M. R. Droop, 1957. The development of artificial media for marine algae. Arch. Microbiol., 25, 392-428
11 Lee, C. H. and D. S. Ha, 1997. Culture characteristics and division process of scuticociliate in vitro. J. Fish Pathol., 10(2), 177-186
12 Ryu, H. Y., J. D. Bang, C. Lee, J. M. Shim and B. S. Kim, 1998. Effects of acute toxicity of chemical treatments on the cultured oliver flounder, Paralichthys olivaceus. J. Aquaculture, 11(2), 223-230   과학기술학회마을
13 吉水 守, 日向進一, 吳明株, 生駒三奈子, 木村喬久, 森 立成, 野 村哲一, 繪面 良男, 1993. ヒラメ(Paralichthys olivaceus) のスク-テイカ感染症.-スク-テイカ 纖毛の培養性狀 .藥劑感受性 . 病原性-. J. Fish Pathol., 6(2), 193-206
14 Parama, A., R. Iglesias, F. Alvarez, J. M. Leiro, J. M. Quintela, C. Peinador, L. Gonzalez, R. Riguera and M. L. Sanmartín, 2004. In vitro efficacy of new antiprotozoals against Philasterides dicentrarchi (Ciliophora, Scuticociliatida). Dis. Aquat. Org., 62, 97-102   DOI
15 乙竹 充, 松里 壽彦, 1986. ヒラメParalichthys olivaceus稚魚 のスク-テイカ纖毛(膜口類)症. Bull. Natl. Res. Aquaculture, 9, 65-68
16 Jung, S. H. and J. W. Kim, 1998. The lethal concentration (LC50) and lethal time (LT50) of formalin on olive flounder fingerlings, Paralichthys olivaceus. Bull. Nat. Fish. Res. Dev. Inst. 54, 125-129
17 Kim, S. M., J. B. Cho, E. H, Lee, S. R. Kwon, S. K. Kim, Y. K. Nam and K. H. Kim, 2004b. Pseudocohnilembus persalinus (Ciliophora: Scuticociliatida) is an additional species causing scuticociliatosis in olive flounder Paralichthys olivaceus. Dis. Aquat. Org., 62, 239-244   DOI
18 Lee, C. H., Y. J. Kang, D. S. Ha and Y. D. Lee, 2001. Occurrence and histopathological observation of scuticociliatosis in the cultured olive flounder, Paralichthys Olivaceus. Bull. Natl. Fish. Res. Dev. Inst. Korea, 59, 68-73
19 Ferugson, H. W., B. D. Hicks, D. H. Lynn and V. E. Ostland, 1987. Cranial ulceration in Atlantic salmon Salmo salar associated with Tetrahymena sp. Dis. Aquat. Org., 2, 191-195   DOI
20 Munday, B. L., P. J. O'Donoghue, M. Watts, K. Rough and T. Hawkesford, 1997. Fetal encephalitis due to the scuticociliate Uronema nigricans in sea-cage, sounthern bluefin tuna Thunnus maccoyii. Dis. Aquat. Org., 30, 17-25   DOI
21 Kim, S. M., J. B. Cho, S. K. Kim, Y. K Nam and K. H. Kim, 2004a. Occurrence of scuticociliatosis in olive flounder Paralichthys olivaceus by Philasterides dicentrarchi (Ciliophora: Scuticociliatida). Dis. Aquat. Org., 62, 233-238   DOI
22 Iglesias, R., A. Parama, M. F. Alvarez, J. Leiro, J. Fernández and M. L. Sanmartín, 2001. Philasterides dicentrarchi (Ciliophora, Scuticociliatida) as the causative agent of scuticociliatosis in farmed turbot Scophthalmus maximus in Galicia (NW Spain). Dis. Aquat. Org., 46, 47-55   DOI
23 Park, I. S., H. B. Kim, M. S. kim and C. W. Park, 1995. Acute toxicity effects of formaline to flounder, Paralichthys olivaceus. J. Fish Pathol., 8(1), 57-67
24 Iglesias, R., A. Parama, M. F. Alvarez, J. Leiro and M. L. Sanmartin, 2002. Antiprotozoals effective in vitro against the scuticociliate fish pathogen Philasterides dicentrarchi. Dis. Aquat. Org., 49, 191-197   DOI
25 Cheung, P. J., R. F. Nigrelli and G. D. Ruggieri, 1980. Studies on the morphology of Uronema marinum Dujardin (Ciliatea: Uronematidae) with a description of the histopathology of the infection in marine fishes. J. Fish Dis., 3, 295-303   DOI