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Evaluation of Effective Breeders Number (Ne) for Stock Enhancement in Olive Flounder Paralichthys olivaceus Using Microsatellite DNA Markers  

Jeong Dal-Sang (Fisheries Resources Enhancement Research Team, NFRDI)
Kim Kwang-Soo (Fisheries Resources Enhancement Research Team, NFRDI)
Kim Kyung-Kil (Fish Genetics and Breeding Research Center, South Sea Fisheries Research Institute, NFRDI)
Publication Information
Journal of Aquaculture / v.19, no.3, 2006 , pp. 205-209 More about this Journal
Abstract
Genetic change from broodstock to hatchery stock of the olive flounder Paralichthys olivaceus and effective number of breeders (Ne) were investigated by the different fertilized-egg collecting methods; E1 (eggs collected one day after spawning) and E2 (eggs collected two days after spawning) using seven microsatellite loci (Kop2, Kop22, Kop18, Kop3, Kop21, Kop9 and Kop26) for the better understanding of stock enhancement. Observed heterozygosity in three stocks ranged from 0.651 at Kop3 to 0.928 at Kop22, with offspring being slightly higher heterozygous over their parents. However, the genetic reduction of offspring was significant. The offspring allele number per locus was reduced to 23.5% for E1 and 17.6% for E2 of their maternal number. Ne to the hatchery stock was estimated to be 21.9 for E1 and 34.3 for E2. The inbreeding coefficients of populations El and E2 were 0.023 and 0.015, respectively. The present study suggests the extension of the egg collection period for a recovery of the genetic diversity in artificially produced offspring.
Keywords
Olive flounder; Microsatellite DNA maker; Genetic variability; Effective number of breeders; Stock enhancement;
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1 Allendorf, F. W., 1986. Genetic drift and the loss of alleles versus heterozygosity. Zoo Biol., 5, 181-190   DOI
2 Hara, M. and M. Sekino, 2003. Efficient detection of parentage in a cultured Japanese flounder Paralichthys olivaceus using microsatellite DNA marker. Aquaculture, 217, 107-114   DOI   ScienceOn
3 Jeong, D. S., 2003. Studies on genetic evaluation of black sea bream Acanthopagrus schlegeli in stock enhancement. Ph. D. thesis, Hiroshima University, Japan, 71 pp
4 Kim, W. J., K. K. Kim, J. H. Lee, D. W. Park, J. Y. Park and J. Y. Lee, 2003. Isolation and characterization of polymorphic microsatellite loci in the olive flounder (Paralichthys olivaceus). Molecular Ecology Notes, 3, 491-493   DOI   ScienceOn
5 Taniguchi, N. and E. Nugroho, 2001. Estimation of effective population size and inbreeding coefficient in hatchery reared red sea bream by microsatellite DNA markers. Fish Genet. Breed Sci., 30, 89-95
6 Tessier, N., L. Bernatchez and JM Wright, 1997. Population structure and impact supportive breeding inferred from mitochondrial and microsatellite DNA analyses in land-locked Atlantic salmon Salmo salar L. Mol. Ecol., 6, 735-750   DOI   ScienceOn
7 Takagi, M., N. Taniguchi, D. Cook, R. W. Doyle, 1997. Isolation and characterization of microsatellite loci from red sea bream and detection in closely related species. Fisheries Sci., 63, 199-204   DOI
8 Norris, A. T., D. G. Bradley and E. P. Cunningham, 2000. Parentage and relatedness determination in farmed Atlantic salmon (Salmo salar) using microsatellite markers. Aquaculture, 182, 73-83   DOI   ScienceOn
9 Fujio, Y., 1997. Genetic variability and heterotic effect within population of aquatic organism. Fish Genet. Breed Sci., 24, 43-52
10 FAO, 1981. Conservation of genetic resources of fish, Report of the expert consultation on the genetic resources of fish. FAO Fish Tech. Paper., 217, 1-43
11 Jeong, D. S., T. Umino, K. Kuroda, M. Hayashi, H. Nakagawa, J. C. Kang, K. Morishima and K. Arai, 2003. Genetic divergence and population structure of black sea bream Acanthopagrus schlegeli inferred from microsatellite analysis. Fish. Sci., 69, 896-902   DOI   ScienceOn
12 Sekino, M., M. Hara and N. Taniguchi, 2002. Loss of microsatellite and mitochondrial DNA variation in hatchery strains of Japanese flounder Paralichthys olivaceus. Aquaculture, 213, 101-122   DOI   ScienceOn
13 Evans, B., J. Bartlett, N. Sweijd, P. Cook and N. G. Elliott, 2004. Loss of genetic variation at microsatellite loci in hatchery produced abalone in Australia (Haliotis rubra) and South Africa (Haliotis midae). Aquaculture, 233, 109-127   DOI   ScienceOn
14 FAO, 1993. Report of the expert consultation on utilization and conservation of aquatic genetic resources. FAO Fisheries Report No. 491, 58 pp
15 Sekino, M., K. Saitoh, T. Yamada, A. Kumagai, M. Hara and Y. Yamashita, 2003. Microsatellite-based pedigree tracing in a Japanese flounder Paralichthys olivaceus hatchery strain: implications for hatchery management related to stock enhancement program. Aquaculture, 221, 255-263   DOI   ScienceOn
16 Perez-Enriquez, R, M. Takagi and N. Taniguchi, 1999. Genetic change and pedigree tracing of a hatchery-reared stocks of red sea bream (Pagrus major) used for stock enhancement, based on microsatellite DNA markers. Aquaculture, 173, 413-423   DOI   ScienceOn