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Evaluation of Effective Breeders Number (Ne) for Stock Enhancement in Olive Flounder Paralichthys olivaceus Using Microsatellite DNA Markers  

Jeong Dal-Sang (Fisheries Resources Enhancement Research Team, NFRDI)
Kim Kwang-Soo (Fisheries Resources Enhancement Research Team, NFRDI)
Kim Kyung-Kil (Fish Genetics and Breeding Research Center, South Sea Fisheries Research Institute, NFRDI)
Publication Information
Journal of Aquaculture / v.19, no.3, 2006 , pp. 205-209 More about this Journal
Abstract
Genetic change from broodstock to hatchery stock of the olive flounder Paralichthys olivaceus and effective number of breeders (Ne) were investigated by the different fertilized-egg collecting methods; E1 (eggs collected one day after spawning) and E2 (eggs collected two days after spawning) using seven microsatellite loci (Kop2, Kop22, Kop18, Kop3, Kop21, Kop9 and Kop26) for the better understanding of stock enhancement. Observed heterozygosity in three stocks ranged from 0.651 at Kop3 to 0.928 at Kop22, with offspring being slightly higher heterozygous over their parents. However, the genetic reduction of offspring was significant. The offspring allele number per locus was reduced to 23.5% for E1 and 17.6% for E2 of their maternal number. Ne to the hatchery stock was estimated to be 21.9 for E1 and 34.3 for E2. The inbreeding coefficients of populations El and E2 were 0.023 and 0.015, respectively. The present study suggests the extension of the egg collection period for a recovery of the genetic diversity in artificially produced offspring.
Keywords
Olive flounder; Microsatellite DNA maker; Genetic variability; Effective number of breeders; Stock enhancement;
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