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Effects of Dietary Supplementation of Spirulina and Astaxanthin for Juvenile Olive Flounder, Paralichthys olivaceus in Low Temperature Season  

Kim Sung-Sam (Faculty of Applied Marine Science, Cheju National University)
Galaz German Bueno (Faculty of Applied Marine Science, Cheju National University)
Lee Kyeong-Jun (Faculty of Applied Marine Science, Cheju National University)
Lee Young-Don (Marine and Environmental Research Institute, Cheju National University)
Publication Information
Journal of Aquaculture / v.19, no.1, 2006 , pp. 57-63 More about this Journal
Abstract
This study was conducted to investigate the effects of dietary supplementation of spirulina (SPI) and asthaxanthin (AST) on growth performance and antioxidant activity in juvenile olive flounder (Paralichthys olivaceus) in low temperature season. Total 180 fish ($27.8{\pm}0.3g$, average weight ${\pm}S.D.$) were randomly divided into 12 groups, and 3 groups were fed one of four isonitrogenous (52% CP) and isocaloric $(18.3\;MJ\;kg^{-1})$ diets containing no SPI and AST, 0.5% SPI, 0.5% AST, and 0.5% each SPI and AST (designated by diets Control, SPI, AST and SPI+AST, respectively). After 6 weeks of feeding trial, the growth performance, feed utilization, whole body composition and survival of fish were not significantly affected by the experimental diets. There were no significant differences in hematocrit, hemoglobin, alanine aminotransferase and aspartate aminotransferase of fish fed all the experimental diets. The DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging assay indicated numerically increased antioxidant activity in liver of fish fed the SPI diet compared to that of fish fed the control diet, even though it was not significant. The present study shows that a low level (0.5%) of dietary supplementation of SPI and/or AST does not affect growth and feed utilization and intake of juvenile olive flounder in low temperature season.
Keywords
Olive flounder; Astaxanthin; Spirulina; DPPH assay;
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