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Post Feeding Trypsin Activity in the Digestive Organs and the Gastric Evacuation Rate of Litopenaeus vannamei (Boone)  

Kim, Su-Kyoung (Crustacean Research Center, Notional Fisheries Research and Development Institute)
Kim, Dae-Hyun (Crustacean Research Center, Notional Fisheries Research and Development Institute)
Kim, Bong-Rae (Crustacean Research Center, Notional Fisheries Research and Development Institute)
Kim, Jong-Seek (Crustacean Research Center, Notional Fisheries Research and Development Institute)
Cho, Yeong-Rok (Crustacean Research Center, Notional Fisheries Research and Development Institute)
Seo, Hyung-Cheol (Crustacean Research Center, Notional Fisheries Research and Development Institute)
Kim, Jong-Hwa (Crustacean Research Center, Notional Fisheries Research and Development Institute)
Han, Chang-Hee (Dong Eui University)
Jang, In-Kwon (West Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
Publication Information
Journal of Aquaculture / v.19, no.1, 2006 , pp. 1-6 More about this Journal
Abstract
The tryptic enzyme activities from hepatopancreas, foregut, midgut and feces were examined to optimize the feeding method in whiteleg shrimp, Litopenaeus vannamei. The highest tryptic enzyme activity was found in hepatopancreas. The enzyme activities of hepatopancreas were 4 times higher than those of foregut per mg dry weight at 30 minutes feeding. Post feeding period, the activities of hepatopancreas increased continuously up to 30 hours after feeding. Trypsin activities of foregut showed about 3 times higher than did those of midgut. Average activity of foregut reached the pick with $303{\pm}68\;(mean{\pm}SE)$ nmol/mg/min at two hours after feeding and kept the activity up to 4 hours after feeding and thereafter the activity decreased. Average tryptic enzyme activity of midgut increased to $96{\pm}26nmol/mg/min$ up to two hours after feeding and it decreased to $52{\pm}17nmol/mg/min$ at five hours after feeding eventhough the gastric evacuation rate was still 50% by then. Foregut clearance occurred in 30 minutes after feeding and midgut weight increased up to 2 hours after feeding. Also we found that the maximal food ingestion in foregut was equivalent to the average 0.3% of its body weight by 30 minutes after feeding. Up to 5 hours after feeding, the weight ratio of midgut to body weight reduced, but still the weight ratio of foregut to body weight kept the similarity until then. These indicated that the tryptic enzyme activity and the clearance rate are different among the digestive organs and between forgot and midgut during the post feeding period in whiteleg shrimp.
Keywords
Whiteleg shrimp; Litopenaeus vannamei; Trypsin; Feeding rate; Evacuation;
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