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Effects of Different Levels of Oxytetracycline on Physiological and Bio- chemical Responses in Olive Flounder, Paralichthys olivaceus  

양정환 (제주대학교 해양과학부, 해양과환경연구소)
여인규 (제주대학교 해양과학부, 해양과환경연구소)
Publication Information
Journal of Aquaculture / v.17, no.4, 2004 , pp. 268-274 More about this Journal
Abstract
Olive flounder was treated with oxytetracycline (OTC) and changes in blood physiology, antioxidant enzymes and heat shock protein (HSP) were recorded to obtain preliminary data for optimal OTC treatment. Blood parameters were measured 1 and 3 h after the OTC treatments at the concentration of 0 (control), 100, 300 and 500 ppm for I h. Hematocrit decreased with time, however the difference was not significant (P>0.05). Reduced number of red blood cell was observed with increasing OTC concentration. Serum glucose level increased as the OTC concentration increased. However, glucose level was similar to control after 3 h. Blood total protein decreased immediately after the OTC treatment but increased after 1 and 3 h. However, the increment in blood total protein was low. Activities of superoxide dismutase enzymes in 300 and 500 ppm groups increased by the OTC concentration. Catalase enzyme activity was negatively affected by the OTC concentration. However, the differences were not significant (P>0.05). High expression of HSP-70 protein was recorded for groups treated with 100 and 500 ppm compared to that of the control group. However HSP-70 mRNA showed a lower increment which was not significant (P>0.05).
Keywords
Olive flounder; Paralichthys olivaceus; Oxytetracycline; Superoxid dismutase (SOD); Catalase (CAT); Heat shock protein (HSP);
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