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Effects of Cryoprotectants and Freezing Rates on Cryopreservation of Catfish, Silurus asotus Sperm  

Kho, Kang Hee (Department of Aquaculture, Yosu National University)
Kang, Kyoung Ho (Department of Aquaculture, Yosu National University)
Publication Information
Journal of Aquaculture / v.17, no.1, 2004 , pp. 8-11 More about this Journal
Abstract
Milt of the catfish was stripped into immobilizing solution containing 175 mM NaCl and 30 mM Tris at pH 7.8 and was successfully cryopreserved after a stepwise freezing procedure. After stepwise thawing, motility of spermatozoa was slightly lower than that of fresh sperm. Batches of 40-80 eggs were fertilized with cryopreserved spermatozoa, after thawing and activation in solution containing 50 mM NaCl, 20 mM Tris and HCl at pH 7.8; this resulted in 62.2% fertilization success, compared to 70.6 % with fresh sperm.
Keywords
Catfish sperm; Cryopreservation; Cryoprotectant; Stepwise freezing;
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  • Reference
1 Motility of pejerrey, Odontesthes bonariensis spermatozoa /
[ Strussmann,C.A.;P.Renard;H.Ling;F.Takasima ] / Fish. Sci.   DOI
2 The freezing of biological systems /
[ Mazur,P. ] / Cryobiology
3 Cryogenic preservation of Atlantic cod, Gadus mothua, sperm /
[ Mounib.M.S.;P.C.Hwang;D.R.Idler ] / J. Fish. Res. Board Can.   DOI
4 Cryopreservation of seabream (Sparus aurata) spermatozoa /
[ Fabbrocini,A.;S.Lubrano Lavadera;S.Rispoli;G.Sansone ] / Cryobiology   DOI   ScienceOn
5 A simple saccharide extender for cryopreservation of rainbow trout, Oncorhynchus mykiss sperm /
[ Holtz,W.;R.Schmidt-Baulatin;M.Meiners-Gefken ] / In: 4th international Sympossium on Reproductive Physiology of Fish
6 Chilled storage of pikey bream, Acanthopagrus berda, sperm and activation in different salinities /
[ Palmer,P.J. ] / Asia. Fish. Sci.
7 La cryconservation de la laitance de la carpe, Cyprinus carpio /
[ Cognie,F.;R.Billard;N.H.Chao ] / J. Appl. Ichthyol.   DOI
8 Cryopreservation of Sarotherodon mossambicus spermatozoa /
[ Harvey,B. ] / Aquaculture   DOI   ScienceOn
9 Cryopreservation of the brown, brook and rainbow trout sperm /
[ Erdahl,D.A.;E.F.Graham ] / Cryo-letters
10 The fertilizing capacity of cryopreserved sharptooth catfish (Clarias gariepinus) sperm /
[ Steyn,G.J.;J.H.Van Vuren ] / Aquaculture   DOI
11 Cryopreservation of fish spermatozoa /
[ Rana,K.J.;J.G.day(ed.);M.R.McLellan(ed.) ] / Methods in Molecular Biology
12 Rise of internal <TEX>&Ca^{2+}&</TEX> accompanies the initiation of trout sperm motility /
[ Cosson,M.P.;P.;J.Cosson;L.Letellier ] / Cell Motil. Cytoskel.   DOI
13 Cryopreservation of spermatozoa of two Asian freshwater catfish, Heteropneutes fossilis and Clarias batrachus /
[ Padhi,B.K.;R.K.Mandal ] / J. Aqua. Trop.
14 Cryopreservation of spermatozoa of the whitefish, Coregonus muksun Pallas /
[ Piironen,J.;H.Hyvarinen ] / J. Fish Biol.   DOI
15 /
[ Zar,J.H. ] / Biostatistical Analysis
16 A review of the biology, handling and storage of salmonid spermatozoa /
[ Scott,A.P.;S.M.Baybes ] / J. Fish Biol.   DOI
17 A comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation /
[ Hara,S.;J.T.Canto;J.M.E.Almendras ] / Aquaculture   DOI
18 Foundation studies in culturing of the Japanese loach, Misgurnus anguillicaudatus: Storing of spermatozoon /
[ Kubota,Z. ] / J. Shimonoseki Coll. Fish.
19 Sperm storage and cross-fertilization of spring and autumn spawning herring /
[ Blaxter,J.H.S. ] / Natyre
20 Study on crogenic preserv ation of grey mullet sperm /
[ Chao,N.H.;H.P.Chen;J.C.Liao ] / Aquaculture
21 Cryopreservation of European catfish, Silurus glanis, sperm /
[ Marian,T.;Z.L.Krasznai;K,Tiews,(ed.) ] / Selection, Hybridization and Genetic Engineering in Aquaculture
22 Cryopreservation of European catfish, Silurus glanis L. spermatozoa /
[ Linhart,O.;R.Billard;J.P.Proteau ] / Aquaculture   DOI   ScienceOn