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Effects of Cryoprotectants on Survival and Hatching of Black Seabream, Acanthopagrus schlegeli Embryos  

임한규 (국립수산과학원 울진수산종묘시험장)
장영진 (부경대학교 양식학과)
조필규 (부경대학교 양식학과)
Publication Information
Journal of Aquaculture / v.16, no.4, 2003 , pp. 262-266 More about this Journal
Abstract
In order to develop a method for the cryopreservation of fish embryos, the determination of optimal concentrations of dimethyl sulfoxide (DMSO), ethylene glycol and glycerol as individual cryoprotectants was performed by using the early embryos of black seabream, Acanthopagrus schlegeli. Optimal concentrations of cryoprotectants were assessed in terms of effects on mortality, median lethal concentration and hatching rate of embryos. The mortality of black seabream embryos immersed in cryoprotectants was related to the concentrations of cryoprotectants and immersion times. The toxicity to embryos was lower in order of DMSO, < ethylene glycol, < glycerol. The results from the mortality, median lethal concentration and hatching rate evaluations suggest that DMSO was the most effective cryoprotectant for black seabream embryos followed by ethylene glycol, and suitable concentrations of DMSO and ethylene glycol were 2.0∼2.25M and 1.0∼1.78M, respectively, with 20 minutes of immersion time.
Keywords
Black seabream; Acanthopagrus schlegeli; Cryoprotectant; Mortality, Hatching rate; LC50;
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