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http://dx.doi.org/10.9766/KIMST.2011.14.4.719

Analysis of Pyrolysis MS Spectra in Top-down Approach and Differentiation of Gram-type Cells  

Kim, Ju-Hyun (ADD)
Publication Information
Journal of the Korea Institute of Military Science and Technology / v.14, no.4, 2011 , pp. 719-725 More about this Journal
Abstract
To apply TMAH-based Py-MS to a field biological detection system for real-time classification of cell-type, reproducible patterns of the TMAH-based Py-MS spectra was known as a critical factor for classification but was seriously disturbed by quantity of cells injected into pyro-tube. This factor is an exterior variable that could not be complemented by improving the performance of the TMAH-based Py-MS instrument. One of idea to solve the knotty problem has been flashed from "Top-down proteomics for identification of intact microoganisms". That is, biomarker peaks are selected from complicate Py-MS spectra for intact microoganisms by tracing out their origins, based on Py-MS spectra for the featured components of different cell-types, in Top-down approach. This idea has been tested in classification of different Gram-type microoganisms. Through the analyses of spectra for the featured components - peptidoglycan and lipoteichoic acid for Gram-positive cells and lipopolysaccharide and lipid A for Gram-negative cells - with comparing to the spectra the corresponding Gram-type cells in the Top-down approach, biomarker peaks were selected to carry out PCA(Principal Component Analysis) in order to see classification of different Gram-types, resulting in significant improvement of their classification. Furthermore, weighting biomarker peaks on intact cell's spectra, based on the data for the featured components of the Gram-types, contributed to elevate classification performance.
Keywords
TMAH(tetramethylammonium hydroxide); Py-MS; Gram-positive Cell; Gram-negative Cell; Biomarker; Principal Component Analysis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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