Browse > Article
http://dx.doi.org/10.3347/kjp.2022.60.5.357

Excretory-Secretory Products of Trichomonas vaginalis Cause Apoptosis in Mouse Sperm in Vitro  

Keum, Jihyun (Deptartment of Obstetrics & Gynecology, College of Medicine, Hanyang University)
Roh, Jaesook (Laboratory of Reproductive Endocrinology, Deptartment. of Anatomy & Cell Biology, College of Medicine, Hanyang University)
Ryu, Jae-Sook (Department of Environmental Biology & Medical Parasitology, College of Medicine, Hanyang University)
Ryu, Ki-Young (Department of Obstetrics & Gynecology, College of Medicine, Hanyang University)
Publication Information
Parasites, Hosts and Diseases / v.60, no.5, 2022 , pp. 357-360 More about this Journal
Abstract
Excretory-secretory products (ESP) of T. vaginalis have been shown to inhibit sperm motility, viability, and functional integrity, leading to a decreased fertilization rate in vitro. This study investigated whether T. vaginalis induce apoptosis and ultrastructural changes of sperm using flow cytometry and electron microscopy. Incubation of sperm with T. vaginalis ESP increased phosphatidylserine externalization and DNA fragmentation, and decreased mitochondrial membrane potential. Transmission electron microscopy of sperm incubated with ESP revealed abnormal features such as distorted heads, broken necks, and acrosomes exocytosis. This is the first report that demonstrates a direct impact of T. vaginalis ESP on sperm apoptosis and architecture in vitro.
Keywords
Trichomonas vaginalis; excretory-secretory products; apoptosis; sperm;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Poole DN, McClelland RS. Global epidemiology of Trichomonas vaginalis. Sex Transm Infect 2013; 89: 418-422. https://doi.org/10.1136/sextrans-2013-051075   DOI
2 El-Shazly AM, El-Naggar HM, Soliman M, El-Negeri M, El-Nemr HE, Handousa AE, Morsy TA. A study on Trichomoniasis vaginalis and female infertility. J Egypt Soc Parasitol 2001; 31: 545-553.
3 Soper D. Trichomoniasis: under control or undercontrolled? Am J Obstet Gynecol 2004; 190: 281-290. https://doi.org/10.1016/j.ajog.2003.08.023   DOI
4 Gopalkrishnan K, Hinduja IN, Anand Kumar T. Semen characteristics of asymptomatic males affected by Trichomonas vaginalis. J In Vitro Fert Embryo Transf 1990; 7: 165-167. https://doi.org/10.1007/BF01135682   DOI
5 Tuttle Jr JP, Holbrook TW, Derrick FC. Interference of human spermatozoal motility by Trichomonas vaginalis. J Urol 1977; 118: 1024-1025. https://doi.org/10.1016/s0022-5347(17)58285-3   DOI
6 Jarecki-Black JC, Lushbaugh WB, Golosov L, Glassman AB. Trichomonas vaginalis: preliminary characterization of a sperm motility inhibiting factor. Ann Clin Lab Sci 1988; 18: 484-489.
7 Hobbs MM, Kazembe P, Reed AW, Miller WC, Nkata E, Zimba D, Daly CC, Chakraborty H, Cohen MS, Hoffman I. Trichomonas vaginalis as a cause of urethritis in Malawian men. Sex Transm Dis 1999: 26: 381-387. https://doi.org/10.1097/00007435-199908000-00003   DOI
8 Roh J, Lim YS, Seo MY, Choi Y, Ryu JS. The secretory products of Trichomonas vaginalis decrease fertilizing capacity of mice sperm in vitro. Asian J Androl 2015; 17: 319-323. https://doi.org/10.4103/1008-682X.145070   DOI
9 Han Q, Liu J, Wang T, Xiao H, Fang Z. Influence of the metabolite produced by Trichomonas vaginalis on human sperm motility in vitro. National J Androl 2004; 10: 272-274 (in Chinese).   DOI
10 Kranjcic-Zec I, Dzamic A, Mitrovic S, Arsic-Arsenijevic V, Radonjic I. The role of parasites and fungi in secondary infertility. Med Pregl 2004; 57: 30-32 (in Serbian). https://doi.org/10.2298/mpns0402030k   DOI
11 Bellamy CO, Malcolmson RD, Harrison DJ, Wyllie AH. Cell death in health and disease: the biology and regulation of apoptosis. Semin Cancer Biol 1995; 6: 3-16. https://doi.org/10.1006/scbi.1995.0002   DOI
12 Grunewald S, Said T, Paasch U, Glander HJ, Agarwal A. Relationship between sperm apoptosis signalling and oocyte penetration capacity. Int J Androl 2008; 31: 325-330. https://doi.org/10.1111/j.1365-2605.2007.00768.x   DOI
13 Zini A, Bielecki R, Phang D, Zenzes MT. Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril 2001; 75: 674-677. https://doi.org/10.1016/s0015-0282(00)01796-9   DOI
14 Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update 2003; 9: 331-345. https://doi.org/10.1093/humupd/dmg027   DOI
15 Jeyendran RS, Van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJ. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 1984; 70: 219-228. https://doi.org/10.1530/jrf.0.0700219   DOI
16 Mielczarek E, Blaszkowska J. Trichomonas vaginalis: pathogenicity and potential role in human reproductive failure. Infection 2016; 44: 447-458. https://doi.org/10.1007/s15010-015-0860-0   DOI
17 Gong YH, Liu Y, Li P, Zhu ZJ, Hong Y, Fu GH, Xue YJ, Xu C, Li Z. A nonobstructive azoospermic patient with Trichomonas vaginalis infection in testes. Asian J Androl 2018; 20: 97-98. https://doi.org/10.4103/1008-682X.195561   DOI
18 Barroso G. Morshedi M. Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod 2000; 15: 1338-1344. https://doi.org/10.1093/humrep/15.6.1338   DOI
19 Sharma R, Masaki J, Agarwal A. Sperm DNA fragmentation analysis using the TUNEL assay. Methods Mol Biol 2013; 927: 121-136. https://doi.org/10.1007/978-1-62703-038-0_12   DOI
20 Sommer U, Costello CE, Hayes GR, Beach DH, Gilbert RO, Lucas JJ, Singh BN. Identification of Trichomonas vaginalis cysteine proteases that induce apoptosis in human vaginal epithelial cells. J Biol Chem 2005; 280: 23853-23860. https://doi.org/10.1074/jbc.M501752200   DOI