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http://dx.doi.org/10.3347/kjp.2021.59.5.439

Expression of Chicken NK-Lysin and Its Role in Chicken Coccidiosis Induced by Eimeria necatrix  

Kim, Woo Hyun (College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University)
Min, Wongi (College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University)
Park, Kwang Il (College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University)
Lillehoj, Hyun S. (Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, ARS, U.S. Department of Agriculture)
Fernandez-Colorado, Cherry P. (Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Banos)
Flores, Rochelle A. (College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University)
Cammayo, Paula Leona T. (College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University)
Nguyen, Binh Thanh (College of Veterinary Medicine & Institute of Animal Medicine, Gyeongsang National University)
Publication Information
Parasites, Hosts and Diseases / v.59, no.5, 2021 , pp. 439-445 More about this Journal
Abstract
Coccidiosis in chickens is an intestinal parasitic disease caused by protozoan parasites named Eimeria spp. In some Eimeria infections, intestinal lymphocytes are known to highly express chicken NK-lysin (cNK-lysin), an antimicrobial peptide with anticoccidial activity. Therefore, this study aims to investigate the expression of cNK-lysin in E. necatrix-infected chickens and its role in E. necatrix infection. The expression of cNK-lysin transcript was significantly increased in E. necatrix sporozoites-treated lymphocytes. In E. necatrix infection, cNK-lysin transcript was induced in intestinal lymphocytes but not in the spleen. The recombinant cNK-lysin exhibited anticoccidial activity against E. necatrix sporozoites as well as immunomodulatory activity on macrophages by inducing proinflammatory cytokines. These results indicated that E. necatrix infection induces high local expression of cNK-lysin and the secreted cNK-lysin helps protect coccidiosis.
Keywords
Eimeria necatrix; chicken; NK-lysin; antimicrobial peptide; coccidiosis;
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