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http://dx.doi.org/10.3347/kjp.2018.56.3.275

Genetic and Morphologic Identification of Spirometra ranarum in Myanmar  

Jeon, Hyeong-Kyu (Department of Parasitology, Parasite Research Center and Parasite Resource Bank, Chungbuk National University School of Medicine)
Park, Hansol (Department of Parasitology, Parasite Research Center and Parasite Resource Bank, Chungbuk National University School of Medicine)
Lee, Dongmin (Department of Parasitology, Parasite Research Center and Parasite Resource Bank, Chungbuk National University School of Medicine)
Choe, Seongjun (Department of Parasitology, Parasite Research Center and Parasite Resource Bank, Chungbuk National University School of Medicine)
Kang, Yeseul (Department of Parasitology, Parasite Research Center and Parasite Resource Bank, Chungbuk National University School of Medicine)
Bia, Mohammed Mebarek (Department of Parasitology, Parasite Research Center and Parasite Resource Bank, Chungbuk National University School of Medicine)
Lee, Sang-Hwa (Department of Parasitology, Parasite Research Center and Parasite Resource Bank, Chungbuk National University School of Medicine)
Sohn, Woon-Mok (Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine)
Hong, Sung-Jong (Department of Medical Environmental Biology, Chung-Ang University College of Medicine)
Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine)
Eom, Keeseon S. (Department of Parasitology, Parasite Research Center and Parasite Resource Bank, Chungbuk National University School of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.56, no.3, 2018 , pp. 275-280 More about this Journal
Abstract
In the present study, we identified a Spirometra species of Myanmar origin (plerocercoid) by molecular analysis using mitochondrial cox1 and nad1 genes, as well as by morphological observations of an adult tapeworm. Spargana specimens were collected from a paddy-field in Taik Kyi Township Tarkwa Village, Yangon, Myanmar in December 2017. A total of 5 spargana were obtained from 20 frogs Hoplobatrachus rugulosus; syn: Rana rugulosa (Wiegmann, 1834) or R. tigrina (Steindachner, 1867). The plerocercoids were used for experimental infection of a dog. After 4 weeks of infection, an adult tapeworm was recovered from the intestine of the dog. Morphologically, the distinct features of Spirometra sp. (Myanmar origin) relative to S. erinaceieuropaei and S. decipiens include a uterine morphology comprising posterior uterine coils that larger than the terminal uterine ball and coiling of the uteri diagonally (swirling) rather than spirally. The cox1 sequences (1,566 bp) of the Myanmar-origin Spirometra species showed 97.9% similarity to a reference sequence of S. decipiens (GenBank no. KJ599679) and 90.5% similarity to a reference sequence of S. erinaceieuropaei (GenBank no. KJ599680). Phylogenetic tree topologies were identical and presented high confidence level of values for the 3 major branches of the 3 Spirometra species in cox1 and nad1 genes. These results indicated that Myanmar-origin Spirometra species coincided with those of S. ranarum and may be considered as a valid species.
Keywords
Spirometra ranarum; spargana; molecular detection; cox1; nad1; Myanmar;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Faust EC, Campbell HE, Kellogg CR. Morphological and biological studies on the species of Diphyllobothrium in China. Am J Epidemiol 1929; 9: 560-583.   DOI
2 McIntosh A. New host records for Diphyllobothrium mansonoides Mueller 1935. J Parasitol 1937; 23: 313-315.
3 Almeida GG, Coscarelli D, Melo MN, Melo AL, Pinto HA. Molecular identification of Spirometra spp. (Cestoda: Diphyllobothriidae) in some wild animals from Brazil. Parasitol Int 2016; 65: 428-431.   DOI
4 Liu Q, Li MW, Wang ZD, Zhao GH, Zhu XQ. Human sparganosis, a neglected food borne zoonosis. Lancet 2015; 15: 1226-1235.   DOI
5 Margono SS, Sutjahyono RW, Kurniawan A, Nakao M, Mulyani T, Wandra T, Ito A. Diphyllobothriasis and sparganosis in Indonesia. Trop Med Health 2007; 35: 301-305.   DOI
6 Liu W, Zhao GH, Tan MY, Zeng DL, Wang KZ, Yuan ZG, Lin RQ, Zhu XQ, Liu Y. Survey of Spirometra erinaceieuropaei spargana infection in the frog Rana nigromaculata of the Hunan Province of China. Vet Parasitol 2010; 173: 152-156.   DOI
7 Liu W, Liu GH, Li F, He DS, Wang T, Sheng XF, Zeng DL, Yang FF, Liu Y. Sequence variability in three mitochondrial DNA regions of Spirometra erinaceieuropaei spargana of human and animal health significance. J Helminthol 2012; 86: 271-275.   DOI
8 Zhang X, Cui J, Wei T, Li LY, Jiang J, Lu JC, Jiang P, Liu LN, Wang ZQ. Survey and genetic variation of Spirometra erinaceieuropaei sparganum in frogs and snakes from Guangxi of southern China. Trop Biomed 2014; 31: 862-870.
9 Zhang X, Wnag H, Cui J, Jiang P, Lin ML, Zhang YL, Liu RD. The phylogenetic diversity of Spirometra erinaceieuropaei isolates from southwest China revealed by multi genes. Acta Trop 2016; 156: 108-114.   DOI
10 Zhang X, Wang H, Cui J, Jiang P, Fu GM, Zhong K, Zhang ZF, Wang ZQ. Characterization of the relationship between Spirometra erinaceieuropaei and Diphyllobothrium species using complete cytb and cox1 genes. Infect Genet Evol 2015; 35: 1-8.   DOI
11 Boonyasiri A, Cheunsuchon P, Suputtamongkol Y, Yamasaki H, Sanpool O, Maleewong W, Intapan PM. Nine human sparganosis cases in Thailand with molecular identification of causative parasite species. Am J Trop Med Hyg 2014; 91: 389-393.   DOI
12 Okamoto M, Iseto S, Shibahara T, Sato MO, Wandra T, Craig PS, Ito A. Intraspecific variation of Spirometra erinaceieuropaei and phylogenetic relationship between Spirometra and Diphyllobothrium inferred from mitochondrial CO1 gene sequence. Parasitol Int 2007; 56: 235-238.   DOI
13 Stamatakis A. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 2006; 22: 2688-2690.   DOI
14 Jongthawin J, Intapan PM, Sanpool O, Sadaow L, Laymanivong S, Thanchomnang T, Maleewong W. Molecular evidence of Spirometra erinaceieuropaei infection in snakes Ptyas korros from Lao PDR and Thailand and frogs Hoplobatrachus rugulosus from Myanmar. Southeast Asian J Trop Med Public Health 2014; 45: 1271-1278.
15 Jeon HK, Park H, Lee D, Choe S, Kim KH, Huh S, Sohn WM, Chai JY, Eom KS. Human infections with Spirometra decipiens plerocercoids identified by morphologic and genetic analyses in Korea. Korean J Parasitol 2015; 53: 299-305.   DOI
16 Meggitt FJ. LXXVII.-On the life history of an amphibian tapeworm (Diphyllobothrium ranarum, Gastaldi). J Nat Hist 1925; 16: 654-655.
17 Lanfear R, Calcott B, Ho SY, Guindon S. Partitionfinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Mol Biol Evol 2012; 29: 1695-1701.   DOI
18 Ronquist F, Huelsenbeck JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003; 9: 1572-1574.
19 Johns GC, Avise JC. A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. Mol Bio Evol 1998; 15: 1481-1490.   DOI
20 Herbert PDN, Ratnasingham S, de Waard JR. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc Biol Sci 2003; 270 (suppl): 96-99.   DOI