Browse > Article
http://dx.doi.org/10.3347/kjp.2017.55.4.451

Characterization of Echinostoma cinetorchis endoribonuclease, RNase H  

Lim, Sung-Bin (Department of Tropical Medicine and Parasitology, Inha University School of Medicine)
Cha, Seok Ho (Department of Tropical Medicine and Parasitology, Inha University School of Medicine)
Jegal, Seung (Department of Tropical Medicine and Parasitology, Inha University School of Medicine)
Jun, Hojong (Department of Tropical Medicine and Parasitology, Inha University School of Medicine)
Park, Seo Hye (Department of Tropical Medicine and Parasitology, Inha University School of Medicine)
Jeon, Bo-Young (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, School of Public Health)
Pak, Jhang Ho (Department of Convergence Medicine, University of Ulsan, College of Medicine, Asan Institute for Life Sciences, Asan Medical Center)
Bakh, Young Yil (Department of Biotechnology, Konkuk University)
Kim, Tong-Soo (Department of Tropical Medicine and Parasitology, Inha University School of Medicine)
Lee, Hyeong-Woo (Institute of Research and Development, Scorpiogen Co.)
Publication Information
Parasites, Hosts and Diseases / v.55, no.4, 2017 , pp. 451-455 More about this Journal
Abstract
Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3'-O-P bond of RNA in a DNA-RNA duplex, producing 3'-hydroxyl and 5'-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.
Keywords
Echinostoma cinetorchis; endoribonuclease; recombinant protein; localization; RNase H;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Seo BS, Cho SY, Hong ST, Hong SJ, Lee SH. Studies on parasitic helminths of Korea. 5. Survey on intestinal trematodes of house rats. Korean J Parasitol 1981; 19; 131-136.   DOI
2 Li WM, Barnes T, Lee CH. Endoribonucleases-enzymes gaining spotlight in mRNA metabolism. FEBS J 2010; 277: 627-641.   DOI
3 Bennett JR. Departments-leading the way-ribonuclease H: a tool in the battle against leishmaniasis. Chem Innov 2001; 31: 3-4.
4 Nowak E, Miller JT, Bona MK, Studnicka J, Szczepanowski RH, Jurkowski J, Le Grice SF, Nowotny M. Ty3 reverse transcriptase complexed with an RNA-DNA hybrid shows structural and functional asymmetry. Nat Struct Mol Biol 2014; 21: 389-396.   DOI
5 Kawahara S, Yamamoto E. Human cases of Echinostoma cinetorchis. Tokyo Iji Shinshi 1933; 2: 1794-1796 (in Japanese).
6 Parniak MA, Min KL, Budihas SR, Le Grice SF, Beutler JA. A fluorescence-based high-throughput screening assay for inhibitors of human immunodeficiency virus-1 reverse transcriptase-associated ribonuclease H activity. Anal Biochem 2003; 322: 33-39.   DOI
7 Yamashita J. Echinostome. Progr Med Parasitol Japan 1964; 1:289-313.
8 Ando R, Ozaki Y. On four new species of trematodes of the family Echinostomatidae. Dobutsugaku Zasshi 1923; 5: 108-119 (in Japanese).
9 Takahashi S, Ishii T, Ueno N. A human case of Echinostoma cinetorchis. Tokyo Iji Shinshi 1930; 2: 1326-1328 (in Japanese).
10 Takahashi S, Ishii T, Ueno N. The second human case of Echinostoma cinetorchis and a case of tapeworm in man. Tokyo Iji Shinshi 1930; 2: 1326-1327 (in Japanese).
11 Takahashi S. The life cycle of Echinostoma cinetorchis and Echinostoma macrorchis, particularly their first and second intermediate hosts. Fukuoka Ika Daigaku Zasshi 1927; 20: 712-723 (in Japanese).
12 Komiya Y. Metacercariae in Japan and adjacent territories. Section II. Special treatise. Progr Med Parasitol Japan 1965; 2: 1-328.