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http://dx.doi.org/10.3347/kjp.2017.55.2.137

Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea  

Sim, Seobo (Department of Environmental and Tropical Medicine & International Healthcare Research Institute, Konkuk University School of Medicine)
Won, Jua (Department of Environmental and Tropical Medicine & International Healthcare Research Institute, Konkuk University School of Medicine)
Kim, Jae-Whan (Department of Environmental and Tropical Medicine & International Healthcare Research Institute, Konkuk University School of Medicine)
Kim, Kyungjin (Department of Environmental and Tropical Medicine & International Healthcare Research Institute, Konkuk University School of Medicine)
Park, Woo-Yoon (Department of Radiation Oncology, College of Medicine, Chungbuk National University)
Yu, Jae-Ran (Department of Environmental and Tropical Medicine & International Healthcare Research Institute, Konkuk University School of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.55, no.2, 2017 , pp. 137-142 More about this Journal
Abstract
Cryptosporidium and Cyclospora are well-known coccidian protozoa that can cause waterborne and food-borne diarrheal illnesses. There have been a few reports regarding contamination in different vegetables with Cryptosporidium, but no data are available regarding the sources of Cyclospora infections in Korea. In the present study, we collected 6 kinds of vegetables (perilla leaves, winter-grown cabbages, chives, sprouts, blueberries, and cherry tomatoes) from July 2014 to June 2015, and investigated contamination by these 2 protozoa using multiplex quantitative real-time PCR. Among 404 vegetables, Cryptosporidium and Cyclospora were detected in 31 (7.7%) and 5 (1.2%) samples, respectively. In addition, Cryptosporidium was isolated from all 6 kinds of vegetables, whereas Cyclospora was detected in 4 kinds of vegetables (except perilla leaves and chives). Cryptosporidium (17.8%) and Cyclospora (2.9%) had the highest detection rates in chives and winter-grown cabbages, respectively. Cryptosporidium was detected all year long; however, Cyclospora was detected only from October to January. In 2 samples (sprout and blueberry), both Cryptosporidium and Cyclospora were detected. Further investigations using TaqI restriction enzyme fragmentation and nested PCR confirmed Cryptosporidium parvum and Cyclospora cayetanensis, respectively. In conclusion, we detected C. cayetanensis in vegetables for the first time in Korea. This suggests that screening should be employed to prevent these protozoal infections in Korea.
Keywords
Cryptosporidium parvum; Cyclospora cayetanensis; vegetable; real-time PCR; Korea;
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