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http://dx.doi.org/10.3347/kjp.2016.54.6.697

Efficacy of Korean Multipurpose Contact Lens Disinfecting Solutions against Acanthamoeba castellanii  

Moon, Eun-Kyung (Department of Medical Zoology, Kyung Hee University School of Medicine)
Park, Hye-Ryun (Department of Parasitology, Dong-A University College of Medicine)
Quan, Fu-Shi (Department of Medical Zoology, Kyung Hee University School of Medicine)
Kong, Hyun-Hee (Department of Parasitology, Dong-A University College of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.54, no.6, 2016 , pp. 697-702 More about this Journal
Abstract
Acanthamoeba keratitis has been increasing in recent years. Main risk factors are contact lens wear and their cleaning solutions. Most contact lens wearers use multipurpose disinfecting solutions (MPDS) for cleansing and disinfecting microorganisms because of its convenience. We determined amoebicidal effects of MPDS made in Korea and their cytotoxicity on human corneal epithelium cells. Fifteen commercial MPDS (A to O) were tested for their amoebicidal effects on Acanthamoeba castellanii trophozoites and cysts by using a most probable number (MPN) technique. Among them, 7 kinds of MPDS showed little or no amoebicidal effects for 24 hr exposure. Solutions A, B, G, H, L, and O showed positive amoebicidal effects, and solutions M and N killed almost all trophozoites and cysts after 24 hr exposure. However, 50%-N solution showed 56% cytotoxicity on human corneal epithelial cells within 4 hr exposure, and 50%-O solution also showed 62% cytotoxicity on human cells within 4 hr exposure. Solution A did not show any cytotoxicity on human cells. These results revealed that most MPDS made in Korea were ineffective to kill Acanthamoeba. The solutions having amoebicidal activity also showed high levels of cytotoxicity on human corneal epithelial cells. New formulations for improved MPDS that are amoebicidal but safe for host cells are needed to prevent Acanthamoeba keratitis.
Keywords
Acanthamoeba castellanii; multipurpose disinfecting solution (MPDS); cytotoxicity;
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1 Stevenson RW, Seal DV. Has the introduction of multi-purpose solutions contributed to a reduced incidence of Acanthamoeba keratitis in contact lens wearers? A review. Cont Lens Anterior Eye 1998; 21: 89-92.   DOI
2 Stehr-Green JK, Bailey TM, Visvesvara GS. The epidemiology of Acanthamoeba keratitis in the United States. Am J Ophthalmol 1989; 107: 331-336.   DOI
3 Seal DV, Kirkness CM, Bennett HG, Peterson M, Keratitis Study Group. Acanthamoeba keratitis in Scotland: risk factors for contact lens wearers. Cont Lens Anterior Eye 1999; 22: 58-68.   DOI
4 Houang E, Lam D, Fan D, Seal D. Microbial keratitis in Hong Kong: relationship to climate, environment and contact-lens disinfection. Trans R Soc Trop Med Hyg 2001; 95: 361-367.   DOI
5 Stiegemeier MJ, Friederichs GJ, Hughes JL, Larsen S, Movic W, Potter WB. Clinical evaluation of a new multi-purpose disinfecting solution in symptomatic contact lens wearers. Cont Lens Anterior Eye 2006; 29: 143-151.   DOI
6 Santodomingo-Rubido J, Mori O, Kawaminami S. Cytotoxicity and antimicrobial activity of six multipurpose soft contact lens disinfecting solutions. Ophthalmic Physiol Opt 2006; 26: 476-482.   DOI
7 Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin Microbiol Rev 2003; 16: 273-307.   DOI
8 Marciano-Cabral F, Puffenbarger R, Cabral GA. The increasing importance of Acanthamoeba infections. J Eukaryot Microbiol 2000; 47: 29-36.   DOI
9 Choy CK, Cho P, Boost MV. Cytotoxicity and effects on metabolism of contact lens care solutions on human corneal epithelium cells. Clin Exp Optom 2012; 95: 198-206.   DOI
10 Zanetti S, Fiori PL, Pinna A, Usai S, Carta F, Fadda G. Susceptibility of Acanthamoeba castellanii to contact lens disinfecting solutions. Antimicrob Agents Chemother 1995; 39: 1596-1598.   DOI
11 Aqeel Y, Siddiqui R, Khan NA. Silencing of xylose isomerase and cellulose synthase by siRNA inhibits encystation in Acanthamoeba castellanii. Parasitol Res 2013; 112: 1221-1227.   DOI
12 Hiti K, Walochnik J, Haller-Schober EM, Faschinger C, Aspock H. Viability of Acanthamoeba after exposure to a multipurpose disinfecting contact lens solution and two hydrogen peroxide systems. Br J Ophthalmol 2002; 86: 144-146.   DOI
13 Turner NA, Russell AD, Furr JR, Lloyd D. Emergence of resistance to biocides during differentiation of Acanthamoeba castellanii. J Antimicrob Chemother 2000; 46: 27-34.   DOI
14 Bowers B, Korn ED. The fine structure of Acanthamoeba castellanii (Neff strain). II. Encystment. J Cell Biol 1969; 41: 786-805.   DOI
15 Beattie TK, Seal DV, Tomlinson A, McFadyen AK, Grimason AM. Determination of amoebicidal activities of multipurpose contact lens solutions by using a most probable number enumeration technique. J Clin Microbiol 2003; 41: 2992-3000.   DOI
16 Yanai R, Yamada N, Ueda K, Tajiri M, Matsumoto T, Kido K, Nakamura S, Saito F, Nishida T. Evaluation of povidone-iodine as a disinfectant solution for contact lenses: antimicrobial activity and cytotoxicity for corneal epithelial cells. Cont Lens Anterior Eye 2006; 29: 85-91.   DOI
17 Moon EK, Kim SH, Hong Y, Chung DI, Goo YK, Kong HH. Autophagy inhibitors as a potential antiamoebic treatment for Acanthamoeba keratitis. Antimicrob Agents Chemother 2015; 59: 4020-4025.   DOI
18 Kong HH, Chung DI. Ultrastructural changes of Acanthamoeba cyst of clinical isolates after treatment with minimal cysticidal concentration of polyhexamethylene biguanide. Korean J Parasitol 1998; 36: 7-13.   DOI
19 Moon EK, Hong Y, Chung DI, Goo YK, Kong HH. Potential value of cellulose synthesis inhibitors combined with PHMB in the treatment of Acanthamoeba keratitis. Cornea 2015; 34: 1593-1598.   DOI