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http://dx.doi.org/10.3347/kjp.2016.54.5.653

Differential Expression of Hox and Notch Genes in Larval and Adult Stages of Echinococcus granulosus  

Dezaki, Ebrahim Saedi (Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences)
Yaghoobi, Mohammad Mehdi (Research Department of Biotechnology, Institute of Sciences and High Technology and Environmental Sciences, Graduate University of Advanced Technology)
Taheri, Elham (Department of Pathology, School of Medicine, Kerman University of Medical Sciences)
Almani, Pooya Ghaseminejad (Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences)
Tohidi, Farideh (Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences)
Gottstein, Bruno (Institute of Parasitology, Faculty of Medicine and Vetsuisse Faculty of the University of Bern)
Harandi, Majid Fasihi (Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences)
Publication Information
Parasites, Hosts and Diseases / v.54, no.5, 2016 , pp. 653-658 More about this Journal
Abstract
This investigation aimed to evaluate the differential expression of HoxB7 and notch genes in different developmental stages of Echinococcus granulosus sensu stricto. The expression of HoxB7 gene was observed at all developmental stages. Nevertheless, significant fold differences in the expression level was documented in the juvenile worm with 3 or more proglottids, the germinal layer from infected sheep, and the adult worm from an experimentally infected dog. The notch gene was expressed at all developmental stages of E. granulosus; however, the fold difference was significantly increased at the microcysts in monophasic culture medium and the germinal layer of infected sheep in comparison with other stages. The findings demonstrated that the 2 aforementioned genes evaluated in the present study were differentially expressed at different developmental stages of the parasite and may contribute to some important biological processes of E. granulosus.
Keywords
Echinococcus granulosus; cystic echinococcosis; HoxB7 gene; notch gene; real-time PCR;
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