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http://dx.doi.org/10.3347/kjp.2016.54.2.225

A Rapid and Convenient Method for in Vivo Fluorescent Imaging of Protoscolices of Echinococcus multilocularis  

Yang, Tao (Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine)
Wang, Sibo (Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine)
Zhang, Xuyong (Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine)
Xia, Jie (Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine)
Guo, Jun (Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine)
Hou, Jixue (Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine)
Zhang, Hongwei (Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine)
Chen, Xueling (Department of Immunology, Shihezi University School of Medicine)
Wu, Xiangwei (Department of General Surgery, First Affiliated Hospital, Shihezi University School of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.54, no.2, 2016 , pp. 225-231 More about this Journal
Abstract
Human and animal alveolar echinococcosis (AE) are important helminth infections endemic in wide areas of the Northern hemisphere. Monitoring Echinococcus multilocularis viability and spread using real-time fluorescent imaging in vivo provides a fast method to evaluate the load of parasite. Here, we generated a kind of fluorescent protoscolices in vivo imaging model and utilized this model to assess the activity against E. multilocularis protoscolices of metformin (Met). Results indicated that JC-1 tagged E. multilocularis can be reliably and confidently used to monitor protoscolices in vitro and in vivo. The availability of this transient in vivo fluorescent imaging of E. multilocularis protoscolices constitutes an important step toward the long term bio-imaging research of the AE-infected mouse models. In addition, this will be of great interest for further research on infection strategies and development of drugs and vaccines against E. multilocularis and other cestodes.
Keywords
Echinococcus multilocularis; fluorescent imaging; in vivo; animal model;
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