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http://dx.doi.org/10.3347/kjp.2016.54.1.71

Influence of 120 kDa Pyruvate:Ferredoxin Oxidoreductase on Pathogenicity of Trichomonas vaginalis  

Song, Hyun-Ouk (Department of Parasitology, School of Medicine, Catholic University of Daegu)
Publication Information
Parasites, Hosts and Diseases / v.54, no.1, 2016 , pp. 71-74 More about this Journal
Abstract
Trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. Iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. However, the exact mechanism was not clarified. In this study, the author investigated whether the 120 kDa protein of T. vaginalis may be involved in pathogenicity of trichomonads. Antibodies against 120 kDa protein of T. vaginalis, which was identified as pyruvate:ferredoxin oxidoreductase (PFOR) by peptide analysis of MALDI-TOF-MS, were prepared in rabbits. Pretreatment of T. vaginalis with anti-120 kDa Ab decreased the proliferation and adherence to vaginal epithelial cells (MS74) of T. vaginalis. Subcutaneous tissue abscess in anti-120 kDa Ab-treated T. vaginalis-injected mice was smaller in size than that of untreated T. vaginalis-infected mice. Collectively, the 120 kDa protein expressed by iron may be involved in proliferation, adhesion to host cells, and abscess formation, thereby may influence on the pathogenicity of T. vaginalis.
Keywords
Trichomonas vaginalis; pyruvate:ferredoxin oxidoreductase; iron; adhesion; abscess formation;
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1 Gorrell TE. Effects of culture medium content on the biochemical composition and metabolism of Trichomonas vaginalis. J Bacteriol 1985; 161: 1228-1230.
2 Ryu JS, Choi HK, Min DY, Ha SE, Ahn MH. Effect of iron on the virulence of Trichomonas vaginalis. J Parasitol 2001; 87: 457-460.   DOI
3 Moreno-Brito V, Yanez-Gomez C, Meza-Cervantez P, Avila-Gonzalez L, Rodriguez MA, Ortega-Lopez J, Gonzalez-Robles A, Arroyo R. A Trichomonas vaginalis 120 kDa protein with identity to hydrogenosome pyruvate:ferredoxin oxidoreductase is a surface adhesin induced by iron. Cell Microbiol 2005; 7: 245-258.
4 Meza-Cervantez P, Gonzalez-Robles A, Cardenas-Guerra RE, Ortega-Lopez J, Saavedra E, Pineda E, Arroyo R. Pyruvate:ferredoxin oxidoreductase (PFO) is a surface-associated cell-binding protein in Trichomonas vaginalis and is involved in trichomonal adherence to host cells. Microbiology 2011; 157(Pt 12): 3469-3482.   DOI
5 Diamond LS. The establishment of various trichomonads of animals and man in axenic cultures. J Parasitol 1957; 43: 488-490.
6 Lehker MW, Arroyo R, Alderete JF. The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis. J Exp Med 1991; 174: 311-318.   DOI
7 Weinberg ED. The role of iron in protozoan and fungal infectious diseases. J Eukaryot Microbiol 1999; 46: 231-238.   DOI
8 Lehker MW, Alderete JF. Iron regulates growth of Trichomonas vaginalis and the expression of immunogenic trichomonad proteins. Mol Microbiol 1992; 6: 123-132.   DOI
9 Griffith E. Iron in biological systems. In Iron and Infection: Molecular, Physiological and Clinical Aspects. Bullen JJ, Griffith E eds. New York, USA. John Wiley and Sons. 1985, pp. 1-25.
10 Tachezy J, Kulda J, Bahnikova I, Suchan P, Razga J, Schrevel J. Tritrichomonas foetus: iron acquisition from lactoferrin and transferrin. Exp Parasitol 1996; 83: 216-228.   DOI
11 Beaver PC, Jung RC, Cupp EW. Clinical Parasitology. 9th ed. Philadelphia, USA. Lea & Febiger. 1984, pp. 49-52.
12 Kim YS, Song HO, Choi IH, Park SJ, Ryu JS. Hydrogenosomal activity of Trichomonas vaginalis cultivated under different iron conditions. Korean J Parasitol 2006; 44: 373-378.   DOI
13 Alderete JF, Lehker MW, Arroyo R. The mechanisms and molecules involved in cytoadherence and pathogenesis of Trichomonas vaginalis. Parasitol Today 1995; 11: 70-74.   DOI
14 Krieger JN, Ravdin JI, Rein MF. Contact-dependent cytopathogenic mechanism of Trichomonas vaginalis. Infect Immun 1985; 50: 778-786.
15 Mendoza-Lopez MR, Becerril-Garcia C, Fattel-Facenda LV, Avila-Gonzalez L, Ruiz-Tachiquin ME, Ortega-Lopez J, Arroyo R. CP30, a cysteine proteinase involved in Trichomonas vaginalis cytoadherence. Infect Immun 2000; 68: 4907-4912.   DOI
16 Demes P, Gombosova A, Valent M, Fabusova H, Janoska A. Fewer Trichomonas vaginalis organisms in vaginas of infected women during menstruation. Genitourin Med 1988; 64: 22-24.