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http://dx.doi.org/10.3347/kjp.2016.54.1.55

Genotype and Phenotype of Echinococcus granulosus Derived from Wild Sheep (Ovis orientalis) in Iran  

Eslami, Ali (Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran (Center of Excellent of Ecosystem and Ultrastructural Changes of Helminthes))
Meshgi, Behnam (Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran (Center of Excellent of Ecosystem and Ultrastructural Changes of Helminthes))
Jalousian, Fatemeh (Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran (Center of Excellent of Ecosystem and Ultrastructural Changes of Helminthes))
Rahmani, Shima (School of Specialized Sciences of Veterinary Medicine, Unit of Sciences and Research, Islamic Azad University)
Salari, Mohammad Ali (School of Specialized Sciences of Veterinary Medicine, Unit of Sciences and Research, Islamic Azad University)
Publication Information
Parasites, Hosts and Diseases / v.54, no.1, 2016 , pp. 55-60 More about this Journal
Abstract
The aim of the present study is to determine the characteristics of genotype and phenotype of Echinococcus granulosus derived from wild sheep and to compare them with the strains of E. granulosus sensu stricto (sheep-dog) and E. granulosus camel strain (camel-dog) in Iran. In Khojir National Park, near Tehran, Iran, a fertile hydatid cyst was recently found in the liver of a dead wild sheep (Ovis orientalis). The number of protoscolices (n=6,000) proved enough for an experimental infection in a dog. The characteristics of large and small hooks of metacestode were statistically determined as the sensu stricto strain but not the camel strain (P=0.5). To determine E. granulosus genotype, 20 adult worms of this type were collected from the infected dog. The second internal transcribed spacer (ITS2) of the nuclear ribosomal DNA (rDNA) and cytochrome c oxidase 1 subunit (COX1) of the mitochondrial DNA were amplified from individual adult worm by PCR. Subsequently, the PCR product was sequenced by Sanger method. The lengths of ITS2 and COX1 sequences were 378 and 857 bp, respectively, for all the sequenced samples. The amplified DNA sequences from both ribosomal and mitochondrial genes were highly similar (99% and 98%, respectively) to that of the ovine strain in the GenBank database. The results of the present study indicate that the morpho-molecular features and characteristics of E. granulosus in the Iranian wild sheep are the same as those of the sheep-dog E. granulosus sensu stricto strain.
Keywords
Echinococus granulosus; wild sheep; genotype; phenotype; Iran;
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1 McManus DP, Bowles J. Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics. Int J Parasitol 1996; 26: 687-704.   DOI
2 Hobbs RP, Lymbery AJ, Thompson RCA. Rostellar hook morphology of Echinococcus granulosus (Batsch, 1786) from natural and experimental Australian hosts and its implications for strain recognition. Parasitology 1990; 101: 273-281.   DOI
3 Chilton NB, Gasser RB, Beveridge I. Differences in a ribosomal DNA sequence of morphologically indistinguishable species within the Hypodontus macropi complex (Nematoda: Strongyloidea). Int J Parasitol 1995; 25: 647-651.   DOI
4 Ahmadi N, Dalimi A. Characterization of Echinococcus granulosus isolates from human, sheep and camel in Iran. Infect Genet Evol 2006; 6: 85-90.   DOI
5 Eslami A, Hoseini SH. Echinococcus granulosus infection of farm dogs of Iran. Parasitol Res 1998; 84: 205-207.   DOI
6 Himsworth CG, Jenkins E, Hill JE, Nsungu M, Ndao M, Thompson RCA, Covacin C, Ash A, Wagner BA, McConnel, A, Leighton FA, Skinner S. Emergence of sylvatic Echinococcus granulosus as a parasitic zoonosis of public health concern in an indigenous community in Canada. Am J Trop Med Hyg 2010; 82: 643-645.   DOI
7 Lavikainen A, Lehtinen MJ, Laaksonen S, Agren E, Oksanen A, Meri S. Molecular characterization of Echinococcus isolates of cervid origin from Finland and Sweden. Parasitology 2006; 133: 567-570.
8 Claridge AW, Mills DJ, Hunt R, Jenkins DJ, Bean J. Satellite tracking of wild dogs in south-eastern mainland Australian Forests: implications for management of a problematic top-order carnivore. Forset Ecol Manag 2009; 256: 814-822.
9 Fasihi Harandi M, Hobbs RP, Adams PJ, Mobedi I, Morgan-Ryan UM, Thompson RCA. Molecular and morphological characterization of Echinococcus granulosus of human and animal origin in Iran. Parasitology 2002; 125: 367-373.
10 Eslami A, Farsad-Hamdi S. Helminth parasites of wild boar, Sus scrofa, in Iran. J Wildl Dis1992; 28: 316-318.   DOI
11 Meshgi B, Eslami A, Bahonar AR, Kharazian-Moghadam M, Gerami-Sadeghian A. Prevalence of parasitic infections in the red fox (Vulpes vulpes) and golden jackal (Canis aureus) in Iran. Iran J Vet Res Shiraz Univ 2002; 10: 387-391.
12 Sadighian A. Helminth parasites of stray dogs and jackals in Shahsavar area, Caspian Sea region. J Parasitol 1969; 55: 372-374.   DOI
13 Hosseini SH, Eslami A. Morphological and developmental characteristics of Echinococcus granulosus derived of sheep, cattle and camel in Iran. J Helminthol 1998; 72: 337-341.   DOI
14 Thompson RCA, McManus DP. Towards a taxonomic revision of the genus Echinococcus. Trend Parasitol 2002; 18: 452-457.   DOI
15 Aminpour A, Hosseini SH, Shayan P. Comparative genotyping of Echinococcus granulosus infecting buffalo in Iran using cox1 gene. Parasitol Res 2011; 108: 1229-1234.   DOI
16 Zhang L, Eslami A, Hosseini SH, McManus DP. Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mitochondrial DNA markers. Am J Trop Med Hyg 1998; 59: 171-174.   DOI
17 Nakao M, McManus DP, Schantz PM, Craig PS, Ito A. A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitology 2007; 134: 713-724.   DOI
18 Moks E, Jogisalu I, Valdmann H, Saarma U. First report of Echinococcus granulosus G8 in Eurasia and a reappraisal of the phylogenetic relationships of' 'genotypes' G5-G10. Parasitology 2008; 135: 647-654.
19 Sharifi I. Seasonal prevalence of hydatid cyst in slaughterhouse of the city of Kerman. Iran J Public Health 1996; 25: 39-46.
20 Daryani A, Alaei R, Arab R, Sharif M, Dehghan MH, Ziaei H. The prevalence, intensity and viability of hydatid cyst in slaughtered animals in the Ardabil province ofIran. J Helminthol 2007; 81: 13-17.
21 Sharbatkhori M, Mirhendi H, Fasihi Harandi M, Rezaeian M, Mohebali M, Eshraghian M, Rahimi H, Bigomkia E. Echinococcus granulosus genotypes in livestock of Iran indicating high frequency of G1 genotype in camel. Experiment Parasitol 2010; 124: 373-379.   DOI
22 Eckert J, Thompson, RCA. Intraspecific variation of Echinococcus granulosus and related species with emphasis on their infectivity to humans. Acta Trop 1997; 64: 19-34.   DOI
23 McManus DP, Bryant C. Biochemistry, physiology and molecular biology of Echinococcus. In Thompson RCA, Lymbery AJ eds, Echinococcus and Hydatid Disease. Walingford, Oxon, UK. CAB International. 1995, pp. 135-181.